Dissertation > Excellent graduate degree dissertation topics show

Effects of Bushenyangjing Decoction on AQP9 Expression in Ovary, Uterus of Rats with PCO

Author: DongHuaJuan
Tutor: ZhongXueMei
School: Luzhou Medical College
Course: Surgery
Keywords: Polycystic ovary The kidney raise by soup Rats AQP9
CLC: R285.5
Type: Master's thesis
Year: 2011
Downloads: 21
Quote: 0
Read: Download Dissertation

Abstract

Objective: To the established sulfate sodium Prasterone rat model of polycystic ovary (polycystic ovary, PCO) objects to study aquaporin 9 (AQP9) and PCO rat ovary, uterus pathological relationship between structural change and observation the Kidney Yang Decoction on PCO rat ovarian, uterine the AQP9 expression of the impact, explore its role in the treatment of PCOS and the possible mechanism of action. Methods: The 23-day-old minor female SD rats 64, were randomly divided into two groups, one for the control group (n = 16), another group of model group (n = 48), sulfate sodium Prasterone, PCO rat model. The success of the model after model rats were randomly divided into model group, high the kidney raise by soup, in the low-dose group and the clomiphene group. Drug intervention is completed, the rats were sacrificed and immunohistochemical SP assay rats in the ovary, uterus AQP9 expression analysis of ovarian granulosa cells, using image analysis software Image-Pro plus 6.0, AQP9 positive expression in endometrial epithelial cells optical density (optical density value, OD), statistical analysis of the differences among the groups. Results: 1 aquaporin 9 (AQP9) in the PCO model group and the normal control group, kidney dependents through soup, in the low-dose group and the clomiphene group granulosa cells and luteal granulosa cells were expressed, immunohistochemistry Coloring is located in the cell membrane and cytoplasm of the particles. Model group AQP9 expression was significantly reduced compared with the control group, the difference was statistically significant (P lt; 0.01); Kidney Yang by soup, in the low-dose group and the clomiphene group AQP9 expression was significantly higher, with the model group compared the differences were statistically significant (P lt; 0.05); raising by the the soup low dose group comparison with the kidney, Kidney Yang by the soup high dose group AQP9 expression was significantly increased, the difference was statistically significant (P lt; 0.01) Kidney Yang by soup dose group AQP9 expression has also increased, but between the difference was not statistically significant (P gt; 0.05) by the soup dose group; Kidney Yang and Kidney Yang after the soup high dose group AQP9 expression was significantly increased, the difference was statistically significant (P lt; 0.01); comparison with clomiphene group, Kidney Yang by soup high dose group AQP9 expression was significantly increased, the difference between the statistical significance (P lt; 0.01) Kidney Yang by soup, the AQP9 the expression of the low-dose group, although increasing, but there was no statistical significance (P gt; 0.05); aquaporin 9 (AQP9) in the PCO model group and the normal control group, kidney raise high by soup, and was expressed in uterine epithelial cells in the low-dose group and the clomiphene group, The immunohistochemical methods positioning endometrial luminal epithelium and glandular epithelial cell membrane and cytoplasm. Model group AQP9 expression was significantly reduced compared with the control group, the difference was statistically significant (P lt; 0.01); Kidney Yang by soup, in the low-dose group and the clomiphene group AQP9 expression was significantly higher, with the model group compared the differences were statistically significant (P lt; 0.05); raising by the the soup low dose group comparison with the kidney, Kidney Yang by the soup high dose group AQP9 expression was significantly increased, the difference was statistically significant (P lt; 0.01) Kidney Yang by soup dose group AQP9 expression has also increased, but between the difference was not statistically significant (P gt; 0.05) by the soup dose group; Kidney Yang and Kidney Yang after the soup high dose group AQP9 expression was significantly increased, the difference was statistically significant (P lt; 0.01); comparison with clomiphene group, Kidney Yang by soup high dose group AQP9 expression was significantly increased, the difference between the statistical significance (P lt; 0.01) Kidney Yang by soup, the AQP9 the expression of the low-dose group, although increasing, but there was no statistical significance (P gt; 0.05). Conclusion: AQP9 expression in the PCO model group granulosa cells and luteal granulosa cells significantly reduced speculated AQP9 may be related to follicular development. AQP9 expression in the the PCO model group endometrial luminal epithelium and glandular epithelial cells significantly reduced speculated AQP9 may be related to endometrial receptivity. Kidney Yang by soup can upregulate model rat granulosa cells and endometrial luminal epithelium and glandular epithelial cells AQP9 expression, possibly through the regulation of follicular development and endometrial receptivity to treat PCOS.

Related Dissertations

CLC: > Medicine, health > Chinese Medicine > Of Pharmacy > Pharmacology > Chinese medicine Experimental Pharmacology
© 2012 www.DissertationTopic.Net Mobile