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A Quantum Dots Fluorescence Bioassay for Detecting Perfluorinated Compounds

Author: ZhangJiong
Tutor: ShuBaiHua;XuShunQing
School: Huazhong University of Science and Technology
Course: Health Inspection and Quarantine
Keywords: Perfluoro compound Quantum dots Fluorescence Biological detection
CLC: R123.1
Type: Master's thesis
Year: 2011
Downloads: 28
Quote: 0
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Abstract


Objective: This study based on the perfluorochemicals the heavily-octane sulfonic acid compounds (PFOS), perfluorooctanoic acid (PFOA) and peroxidase value-added activated receptor (PPARα) specific binding characteristics and novel fluorescent markers quantum dots ( QDs) to establish an entirely new for environmental waters PFCs fast, high-throughput, fluorescence detection methods of molecular biology. Methods: Preparation of the PFOS-BSA complex and the package is in the microtiter plate, activation of PPARα with a fixed PFOS competition standard was added to each concentration group PFOS, after the transfer of the composite body of the PPARα-PFOS specific antibodies to the package is PPARα fixed in microtiter plates. Biotin-labeled DNA probe was added to the microtiter plates, then QDs as fluorescent markers constitute specific quantum dot fluorescent probes in the PPRE DNA duplexes, labeled avidin - biotin system, and finally through detect binding PFOS activated PPARα receptor signal intensity of the fluorescent probe to quantitative detection of PFOS, after the standard curve based on the fluorescence intensity and the corresponding experimental group PFOS concentrations, using this method is the same on PFOA detected. In environmental water samples collected in accordance with the national standards and pretreatment, respectively, using the above method and high performance liquid chromatography-mass spectrometry (HPLC / MS) method for detection, based on the test results and standard curve derived concentration of PFCs in environmental water samples. Results: Using the the QDs fluorescence biological testing method by measuring different concentrations of PFOS and PFOA standard, the change of a signal of detection QDs, and draw out the standard curve (PFOS: Y = 19.112x-23.995 of R ~~ 2 = 0.9642; of PFOA: y = 17.601 x-20.914, R ~ 2 = 0.973), results showed that the method at a concentration of 2.5 ng/L-75 ng / L linear range of the best and with good reproducibility, the detection limit of 2.5 ng / L, spiked The recoveries were 107.8% and 110.3%, according to the test results of different concentrations of standard peak area to draw the standard curve (PFOS: y = 2E-05x-26.479 of R ~~ 2 = 0.996; of PFOA: y = 2E-05x-25.023 R ~ 2 = 0.997), and results showed good reproducibility of the method has a concentration of 2.5 ng/L-75 ng / L range, the lowest detection limit of 1 ng / L, the recoveries of 104.3% and 105.9%, respectively. PFCs concentration in the the Yangtze (Wuhan section), the Han River (Wuhan section), East Lake, Wuhan City, a waterworks, and a brand of bottled drinking water, water samples using two methods for testing the consistency of test results of the two methods . Conclusion: QDs the fluorescent biological detection method has obvious advantages in the actual environmental water samples PFCs detection, both have quick and easy method of immunofluorescence, and through the application of novel fluorescent markers of QDs, relative to traditional fluorescence techniques improve The sensitivity and accuracy, saving time and economic costs, and there are broad prospects for application in the screening of large sample size.

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CLC: > Medicine, health > Preventive Medicine,Health > Environmental health,environmental medicine > Water and water supply sanitation > Health surveillance of the health standards of water and water
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