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An Experimental Study of Inhibition the Expression of Ppif Gene Reduce Renal Ischemia-reperfusion Injury in Rats

Author: MaJieFeng
Tutor: ChenZhiQiang
School: Huazhong University of Science and Technology
Course: Surgery
Keywords: Ppif gene Lentiviral vectors Renal ischemia-reperfusion injury Apoptosis
CLC: R363
Type: Master's thesis
Year: 2011
Downloads: 4
Quote: 0
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Abstract


Objective inhibit Ppif gene expression observed protective effect on rat ischemia -reperfusion injury in the kidney from the analysis , to discuss its role in principle . First established using rat renal ischemia-reperfusion model and random experimental rats grouping ( group 3 ) : blank control group (NC group ) , negative control group (CON Group ) , the treatment group (Treated Group) ( per groups of 20 rats each ). Reperfusion treated rats the injection Ppif gene targeting RNAi lentiviral vector dose of 0.3 mL ( 4μg / g ) , the negative control group injected with 0.3 mL of saline reperfusion (including the volume fraction of 0.01 DMSO that dimethyl sulfoxide ) , blank control group does not deal with the renal pedicle , that is the kidneys not experienced ischemia-reperfusion , the remaining measures of the negative control group . Apoptosis index ( AI ) of each experimental group of rats after a predetermined time , respectively , with the corresponding method detection level of serum creatinine (Cr) , blood urea nitrogen (BUN) level , HE staining cells pathological examination. Results treated rats relative to the negative control group rats , the determination of Cr and BUN values ??were significantly decreased in the blood , AI was significantly lower , both differences were statistically significant (P lt; 0.05). The results of pathological examination (HE tissue staining method) : rats contrast , treatment of rats ischemia significantly reduced . The conclusion rat Ppif gene expression can be Ppif gene targeted RNAi lentiviral vector effectively suppressed , so as to achieve the purpose of inhibiting the mitochondrial pathway of apoptosis , eventually play ischemic kidney when renal ischemia reperfusion The protective effect .

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