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Objective: To successfully established disseminated Fusarium infection in a mouse model to explore the Fusarium in BALB / c mice pathogenicity. Observed in different immunization status Fusarium induced disseminated Fusarium infection disseminated infection conditions and organ case. Methods: The experimental animals were randomly divided into five groups, specifically grouped as follows: the experimental group A (12 only): ten normal mice immunized intraperitoneally inoculated bacterial suspension 0.5ml (1 × 106cfu/animal); experimental group B (12 only): immunocompetent mice inoculated with bacterial suspension 0.5ml (5 × 107cfu/animal); experimental group C (12 only): immunosuppressed mice inoculated with bacterial suspension 0.5ml (1 X 106cfu/animal); experimental group D ( 12): immunosuppressed mice inoculated with bacterial suspension 0.5ml (5 × 10'cfu / animal); control group E (12 only): immunocompetent mice by intraperitoneal injection of an equal volume of saline 0.5ml. Mice were observed daily symptoms (loss of appetite, mental health, weight, hair, survival days), were observed for 28 days. The end of death and observation (ie the 28th) mice dissected organs (heart, kidneys, lungs, liver, spleen, brain tissue) OK fungal culture and histological examination (HE staining and PAS staining). Take each group of mice blood line fungal culture. Results 1 after infection of the general condition and survival time: after intraperitoneal inoculum experiment A, B group and E group activities and food intake in mice compared with no significant change before the inoculum. Groups C and D experiments with varying degrees eating, reduced activity, weight loss, hair fall against the light, and D activities and eating mice was significantly reduced compared with C group. Mortality of mice in each group were A, B, E group were 0% (0/12), C group was 16.7% (2/12), D group was 100% (12/12) 2, fungal culture : Immune normal group (A, B group) and the control group E group organs fungal cultures were negative, blood cultures were all negative. Immunosuppression group (C, D group) were positive fungal culture. 3, histopathology: C group and D group organs HE staining showed infiltration of inflammatory cells, blood vessels dilate, tissue necrosis and other pathological changes. PAS staining: spleen seen more purple mycelium, the shape of the roots to the splenic parenchyma invasion. Heart and liver: see scattered purple hyphae. 4, 100% of immunosuppressed mice infected organs of mice immunized control group showed no infection. Conclusion 1 Fusarium is an opportunistic pathogen, can cause immunosuppression or immune-deficient mice infected organs. Organ infection common pathological features of inflammatory cell infiltration, vascular dilation, tissue degeneration and necrosis. 2, inoculated intraperitoneally with Fusarium can cause immune suppression in mice with disseminated infection. 3, Fusarium can affect the spleen, liver, heart, kidney, lungs, brain, spleen may be the most vulnerable to the invasion of organs.
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