Dissertation > Excellent graduate degree dissertation topics show

Cloning and Fuctional Study of clp Gene of Xanthomonas Oryzae

Author: HanZhiCheng
Tutor: LiuFengQuan
School: Nanjing Agricultural College
Course: Plant Pathology
Keywords: Xanthomonas oryzae clp gene Pathogenicity Motility Flagellar activity Chemotaxis Extracellular polysaccharide Virulence
CLC: S435.111.4
Type: Master's thesis
Year: 2010
Downloads: 3
Quote: 0
Read: Download Dissertation

Abstract


Xanthomonas oryzae pv. oryzicola (Xooc) and Xanthomonas oryzae pv. oryzae (Xoo) cause leaf streak and leaf blight on rice, respectively. In this study, we identified and characterized the contribution of clp gene to motility, chemotaxis, extracellular polysaccharide (EPS) and virulence of the phytopathogens X. oryzae pv. oryzae strain PXO99 and X. oryzae pv. oryzicola strain Rs105.(I) A clp homologue, designated clprs, was cloned from X. oryzae pv. oryzicola, with degenerated primers by polymerase amplification reaction (PCR). A clp deletion mutant of strain Rs105 was constructed, and confirmed by PCR. Compared to the wild-type strain Rs105, the mutation of clp did not affecte growth, the clp mutant could yet elicit hypersensitive response (HR) on nonhost plants tobacco. Interestingly, the clp mutant do not lacked detectable flagella, but highly impaired in motility. Furthermore, the clp mutant displayed almost no chemotaxis toward glucose under aerobic conditions. Remarkably, it was observed that the clp mutant exhibited reductive production of extracellular polysaccharide (EPS) and significant reduction of virulence on adult rice plants compared to the wildtype strain. Our findings indicated that the clp gene played an important role in virulence on rice.(II) A clp homologue, designated clpxoo, was cloned from Xanthomonas oryzae pv. oryzae strain PXO99, with degenerated primers by PCR. Sequence comparison revealed that the gene in Xanthomonas is relatively conservative. A clp deletion mutant of strain PXO99 was constructed, and confirmed by PCR. Compared to the wild type, motility and chemotaxis of the clp gene mutant was decreased, and the deposit in a test tube experiments significant differences. But, we found that the flagellum of the clp mutant and the wild type were similar. It was observed that the clp mutant exhibited reductive production of EPS. By using leaf-cutting, susceptible host IR24 was inoculated, the clp mutant reduced pathogenicity. But, like the wild-type strain, the mutant strain could also stimulate HR in nonhost tobacco. Speculate the clp gene has effected on virulence, flagella and chemotaxis of Xanthomonas oryzae pv, oryzae.

Related Dissertations

  1. Functional Analysis of Proteins Encoded by RNA2 of Wheat Yellow Mosaic Virus,S435.121
  2. Isolation and Identification of Avian Pathogenic Escherichia Coli from Chicken and Characterizations of Its Virulence-Associated Genes,S852.61
  3. Transcriptional Regulation of Genes Encoding MFS by LysR in Vibrio Cholerae,R378
  4. The Influence of Antibiotics on Yogurt During the Fermentation,TS252.54
  5. Cloning and Function Analysis of crp and Tatc Gene from Erwinia Amylovora Strain Ncppb1665,S436.612
  6. A Study of the Isolation and Identification of Fusarium Wilt Germ in Sesame and the Characteristics of Sesame Germplasm Anti-Fusarium Wilt Disease,S435.653
  7. Cloning and Functional Analysis of Acidovorax Avenae Subsp. Citrulli Partial hrp Gene Cluster,S436.5
  8. Screening of Pathogenicity-Ralated Genes and Function Analysis of luxR Gene in Acidovorax Avenae Subsp. Citrulli,S436.5
  9. Functional Analysis of Inhibitor of Apoptosis Protein Gene Cpbirl in Cryphonectria Parasitica,S436.64
  10. Characterization of Two Component System MoSLN1 and MoSKN7 in Development and Pathogenicity of Magnaporthe Oryzae,S435.111.41
  11. Identification and Fuctional Study of Genes Induced in Xanthomonas Oryzae PV. Oryzae by Its Host Plant,S435.111.4
  12. Functional Analysis of the Flagellar Genes and Asparagine Synthetase B Gene Regulated by DSF in Xanthomonas Oryzae PV.Oryzicola,S435.111.4
  13. Genetic Structure of the Wheat Powdery Mildew Populations from Henan, Hubei and Chongqing Province (City),S435.121
  14. Some Pharmacology and Toxicity of Three Prescriptions from Chinese Veterinary Pharmacopoeia,S859.5
  15. Molecular Characterization and Pathogenicity for Poultry of Eleven H5N2 Avian Influenza Viruses in China,S852.65
  16. Functional Analysis of Inhibitor of Apoptosis Protein Gene Cpbir1 in Cryphonectria Parasitica,S436.64
  17. Secreening of Harpin-like Genes and Identification of Extracellular Polysaccharide-Associated Genes in Xanthomonas Oryzae Pv. Oryzicola,S435.111.42
  18. Analysis of Resistance in Transgenic Rice with OsDUF500 Gene to Xanthomonas Oryzae Pv. Oryzae and Drought Tolerane in Transgenic Tobacco with OsWRKY30 Gene,S511
  19. Virulence Factors of Enterococcus Faecalis Expression in the Biofilm of Starvation Phase and after Two Root Canal Conventional Drugs,R780.2
  20. Edwardsiella Tarda Infection on Andrias Davidianus and the Protection of Dead Vaccine at Primary Immune,R392.1

CLC: > Agricultural Sciences > Plant Protection > Pest and Disease Control > Crop pests and diseases and their prevention > Cereal crop pests and diseases > Rice pests and diseases > Disease > Transgression ( pass ) an infectious agent harmful
© 2012 www.DissertationTopic.Net  Mobile