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Objective: To observe diabetic nephropathy associated with hypertensive rat kidney tissue salt-inducible kinase 1 (Salt-inducible kinase 1, SIK1), Na ~ K to-ATPaseα1 of expression and sodium-potassium ATPase enzyme activity observed staurosporine ( staurosporine) to SIK1 expression gliquidone associated with the protective effect of hypertension on diabetic nephropathy and on SIK1 expression regulation. Method: by the high-fat diet combined with 45 mg / kgSTZ type 2 diabetic rat model induced by intraperitoneal injection. Male Wistar rats were randomly divided into normal control group (10), model group (10), staurosporine (9) and gliquidone treatment group (9), the administration of 12 consecutive weeks. Observing fasting blood glucose (FBG), postprandial blood glucose (PBG), lipids (TG, TC), serum insulin (FINS), 24h urine output, serum creatinine (Scr), urine creatinine (Ucr), urine microalbumin (UAE ), and insulin sensitivity index (ISI) and endogenous creatinine clearance rate (Ccr), simultaneous detection of rat tail artery blood pressure. Rats were killed, observed kidney weight index (KI), HE staining of pathological changes of the kidneys; detection SIK1 protein expression by immunohistochemistry method; detection SIK1, sodium-potassium ATPase enzyme α1 mRNA expression by RT-PCR method; simultaneous detection of kidney sodium potassium ATP activity. Results: 1 high-fat diet for 4 weeks, compared with the normal control group, the model rats TG, TC, FINS significantly increased (P lt; 0.05, P lt; 0.01), the ISI significantly reduce (P lt; 0.05), FBG difference had no statistical significance (P gt; 0.05); model rats after one week of STZ injection, FBG was significantly higher and the difference was statistically significant (P lt; 0.01). Compared with the normal control group, model group, FBG, PBG, TG and the TC were significant with increased (P lt; 0.01); compared with the model group, staurosporine group FBG, PBG, the TC are lowering (P lt; 0.01 or P lt; 0.05), TG difference had no statistical significance (P gt; 0.05); the gliquidone rats FBG, TG, TC were lower (P lt; 0.01 or P lt; 0.05), PBG difference was not statistically significant (P gt; 0.05). 3. Compared with the normal control group, model group, the blood pressure from molded after six weeks began to increase, continuing to 12 weeks (P lt; 0.05); compared with the model group, the rats of the the staurosporine group and gliquidone treatment blood pressure were lower (P lt; 0.05). Compared with the normal control group, model group Ccr significantly with lowering (P lt; 0.01), UAE, KI significantly higher (P lt; 0.01); compared with the model group, staurosporine group and glibenclamide methaqualone treatment group Ccr significantly with increased ( P lt; 0.01), UAE, KI significantly lower (P lt; 0.05). 5. Pathological results show: the glomerular capillary loop open normal rats, normal tubular structure; model rats increases varying degrees of glomerular glomerular cysts wider, mesangium area broadening, thickening of the glomerular basement membrane (GBM), tubular epithelial cell swelling, the tubular luminal narrowing, renal interstitial inflammatory cell infiltration; the staurosporine group and gliquidone glomerular and tubular reduce the degree of swelling, basement membrane thickening improved pathological changes of varying degrees of reduction. Compared with the normal control group, model group rat kidney tissue SIK1 mRNA in SIK1 protein expression significantly with increased (P lt; 0.01), of Na ~-K of ~-ATPaseα1 mRNA expression significantly with increased (P lt; 0.01) and sodium-potassium ATP activity was significantly increased (P lt; 0.01); compared with the model group, staurosporine group SIK1 mRNA, the SIK1 protein and sodium potassium ATPase activity differences had no statistical significance (P gt; 0.05), gliquidone the groups above indicators were lower (P lt; 0.01, P lt; 0.05). Conclusion Type 2 diabetic nephropathy associated with elevated hypertension rat model of renal tissue SIK1 mRNA and protein expression, may itself or by adjusting the sodium-potassium ATPase activity, and diabetic nephropathy with hypertension disease-related; Staurosporine on SIK1 express no inhibition; gliquidone group SIK1 mRNA and protein, Na the ~~-K ~~-ATPaseα1 mRNA and sodium potassium ATPase enzyme activity decreased in both treatment groups biochemical indicators and improvement in renal pathology.
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