Dissertation > Excellent graduate degree dissertation topics show

Department of Respiratory Medicine, Union Hospital of Tongji Medicine College, Huazhong University of Science and Technology

Author: ShenDaHong
Tutor: TaoXiaoNan
School: Huazhong University of Science and Technology
Course: Department of Respiratory Medicine
Keywords: Rat alveolar macrophages Bleomycin Kv channel Pulmonary fibrosis
CLC: R563.9
Type: Master's thesis
Year: 2011
Downloads: 13
Quote: 0
Read: Download Dissertation

Abstract


Objective To study the the bleomycin rat alveolar macrophage cell line NR8383 cells Kv1.3 and Kv1.5 channel expression . Methods Cultured rat macrophages NR8383 cells , and then (1) the cells were divided into five groups , and processing by the five kinds of concentrations BLM ( 0,0.1,1.0,10,100 mU / ml ) ; (2) The cells were divided into four groups (① NS group ; ② DEX group ; ③ DEX BLM group ; ④ B LM group) , the corresponding administration processing . Application Realtime PCR and Western blot analysis were used to detect Kv1.3 and Kv1.5 channel expression at the mRNA and protein levels . Kv1.3 and Kv1.5 channel Results 1.NR8383 cells expressed both at the mRNA and protein levels . 2 compared with the control group , the BLM stimulated NR8383 cells Kv1.5 channels at the mRNA and protein levels increase, there is a statistically significant ( P lt; 0.05 ) . Conclusion alveolar macrophages Kv channel may be involved in bleomycin-induced pulmonary fibrosis .

Related Dissertations

  1. Osthol on bleomycin- induced pulmonary fibrosis in rats and its mechanism of,R563.9
  2. The Dynamic Changes of Serum Cytokines in Paraquat Poisoning Rats and the Artesunate Intervention Studies,R595.4
  3. Effect of Xuebijing on Lung Fibrosis in a Rat Model of Oleic Acid-induced Lung Injury,R563.8
  4. The Effects of Rosiglitazone and Prednisone on the Expression of FIZZ1 in the Pulmonary Fibrosis Induced by Blemycin,R965
  5. Change of UGBP in Lung Fibroblasts and Human Bronchial Epithelial and Primary Mechanism,R563
  6. Nrf2, γ-GCS expression matrine bleomycin- induced pulmonary fibrosis,R285.5
  7. Beidan Yifei on Angiogenesis in Rats with Idiopathic Pulmonary Fibrosis Effects of ET-1 and VEGF,R285
  8. Effect of N-acetyl-seryl-aspartyl-lysyl-proline on the Proliferation and Collagen Synthesis in Cultured Rat Pulmonary Fibroblasts Through JNK Signal Transduction Mediated by TGF-β1,R563
  9. Effect of Angelica Polysaccharide on Pulmonary Function Extracellular Stroma and Pathological Morphology of Rats with Lung Fiberosis,R285.5
  10. The Effectiveness of Cultured Cordyceps Sinensis on Pulmonary Fibrosis Induced by Bleomycin in Rats,R285.5
  11. Caveolin-1 Expression and significance of pulmonary fibrosis,R575.2
  12. The Effect of Rosiglitazone on the Changes of TGF-β1/JNK Signal Pathway Expression in OLETF Rats Lung,R965
  13. Imaging Study about Distribution Sites of Lesion Areas of Pulmonary Fibrosis Induced by Paraquat,R595.4
  14. Investigation on the Expression Changes and Intervention of TGF-β1/JNK Signaling Pathway in OLETF Rats Lung,R587.1
  15. Rosiglitazone and dexamethasone on bleomycin -induced pulmonary fibrosis Caveolin-1 expression,R563
  16. PPARγ Agonist and Dexamethasone Alleviate the Pulmonary Fibrosis Induced by Bleomycin Through Upregulating Pulmonary Glucocorticoid Receptors in Rats,R563
  17. Role of Serum Amyloid P Component on Hyperoxia-inducedlung Fibrosis and Construction of the Recombinat Adenovirus of AD5-SAP,R722.1
  18. The Effect of Apigenin on Cell Proliferation and Toxicity in Response to Therapeutic Drugs in Non-Small Cell Lung Cancer Cells,R734.2
  19. Dynamic Expression and Significance of NF-κB and ICAM-1 in the Rats of Bleomycin-induced Pulmonary Fibrosis.,R563.9
  20. Basic Research on the Injury of Lungs about Paraquat Poisoning Rats and the Optimum Therapeutic Dose of Ulinastatin,R595.4

CLC: > Medicine, health > Internal Medicine > Respiratory system and chest diseases > Pulmonary disease > Other
© 2012 www.DissertationTopic.Net  Mobile