Dissertation > Excellent graduate degree dissertation topics show

The Effect of Ang(1-7)/MAS Pathway on Pancreatic Islet Microcirculation and Function in Rat with Long-term High-fat Diet

Author: LiGuangLi
Tutor: YuanLi
School: Huazhong University of Science and Technology
Course: Internal Medicine
Keywords: Angiotensin-(1-7 ) Islet function Insulin Resistance Islet endothelial function Pancreatic microcirculation
CLC: R587.1
Type: Master's thesis
Year: 2011
Downloads: 14
Quote: 0
Read: Download Dissertation

Abstract


Objective Ang (1-7) and the MAS receptor blocking agent A-779 intervention long-term high-fat high-calorie feeding induced insulin resistant rats, observed Ang (1-7) / MAS pathway of islet endothelial cells, islet microcirculation and islet function, and to investigate the regulatory role of long-term high-fat induced islet β-cell dysfunction and related mechanisms. 80 male Wistar rats basic feed adaptive feeding two weeks, 20 were randomly selected as the normal control group (NC group, n = 20), given the normal diet, the remaining 60 rats high fat and high calorie diet feeding randomly divided into high-fat group (H0, n = 20) after 12 weeks, the intervention group of Ang-(1-7) (H1 group, n = 20), Ang (1-7) A-779 intervention group (H2, n = 20) were treated with saline, Ang-(1-7), Ang-(1-7) A-779 subcutaneous injection four weeks, the intervention dose of Ang-(1-7) and A-779 are 300μg · kg -1 · d -1 . The colored microspheres Technology (sedimentation) detection of pancreatic microcirculation blood flow; immunofluorescence assay islet of vWF (von Willebrand factor) expression and relative insulin content in pancreatic β-cells (insulin relative concentration IRC); RT-PCR assay islet endothelial cell markers mRNA expression levels of vWF (von Willebrand factor); intravenous glucose tolerance test (IVGTT) and intravenous insulin release the test (IVIRT) detection reflect β-cell function. The results compared with the NC group, the the H0 group of the glucose infusion rate (GIR) decreased by 32% [(5.19 ± 0.81 vs 7.65 ± 0.45) mg · kg -1 · min -1 -1 · min -1 P lt; 0.05); give Ang (1-7) after the intervention, compared with H0, H1 rats glucose infusion rate (GIR) increased 29% (6.72 ± 0.39 vs 5.19 ± 0.81) mg · kg 17.52 vs 42.25 ± 14.39, P lt; 0.05), immunofluorescence results showing the amount of β-cell insulin expression was significantly higher (P lt; 0.05), pancreas micro-circulation blood flow increased 46% [(0.866 ± 0.110 vs 0.595 ± 0.062) vs 13.8 ± 2.21 P lt; 0.05), microvascular density was significantly increased, the islet vWFmRNA expression levels of 35% (P lt; 0.05); give MAS receptor blocker, compared with H1, H2 glucose infusion rate , acute insulin secretion response (AIR) decreased 23% (50.60 ± 14.39 vs 65.65 ± 17.52, P lt; 0.05), β-cell insulin expression amount substantially significantly lower (P lt; 0.05), pancreatic micro-circulation of blood flow to reduce by 35% [ % (relative concentrations were 14.4 ± 2.85 vs 19.3 ± 3.16 P lt; 0.05), islets vWFmRNA expression levels decreased 15% (P lt; 0.05). Conclusion Long-term high-fat high-calorie feeding-induced insulin resistance in rats the pancreatic microcirculation perfusion decreased slightly, the islet microcirculation the decompensated performance: endothelial cell dysfunction, reduced microvessel density. Ang (1-7) combined to improve the islet endothelial function mediated through its receptor MAS islet microcirculation, thus improving the long-term high-fat high-calorie diet rat islet function.

Related Dissertations

  1. Study on Relationship between Endothelin-1, Insulin Resistance and Acute Ischemic Cerebrovascular Disease,R743.3
  2. Expression and Significance of HIF-1α on the Insulin Resistance during the Injury of Reperfusion of Ischemic Myocardium in Dog Undergoing Cardiopulmonary Bypass,R654.1
  3. Shamisen Medicinal and Edible Herbs on glucose and lipid metabolism in the metabolic syndrome and antioxidant effects,R285.5
  4. Individuals with type 2 diabetes, serum complement factor H, hs-CRP levels and insulin resistance study,R587.1
  5. Osthole Improves Insulin Resistance and Its Possible Mechanisms in Fatty Liver Rats,R285.5
  6. The Expression of TAFI in the Patient with Polycystic Ovary Syndrome,R711.75
  7. Effect of Whey Protein on Improving Insulin Resistance and the Underlying Mechanisms,R587.1
  8. Effect of Intensive Insulin Therapy on Resting Energy Expenditure in Postoperative Patients Underwent Radical Distal Gastrectomy,R735.2
  9. Efects of Obesity on Insulin Resistance, Endothelial Function and β Cell Function,R587.1
  10. The Relationships among Osteopontin, Obesity and Insulin Resistance,R589
  11. Ang(1-7) Improves Pancreatic Islets Function Via Upregulation of eNOS Expression in Rats with High-fat Diet,R587.1
  12. Effects of Berberine on 11β-hydroxysteroid Dehydrogenase Type 1 mRNA Expression in Insulin-resistant HepG2 Cells,R587.1
  13. 1. Improvement of tmTNF-α in Insulin Resistance of Adipocytes and Its Underlying Mechanism. 2. Construction of Conserved Sequence Deleted TNF-α Mutant and the Trimerization of Remodeled TNFRBP.,R587.1
  14. The Expression of microRNA in the Ovary of Polycystic Ovary Syndrome Rat Model,R711.75
  15. PEF AT2R mediated gene expression in the vascular localized impact on neointimal,R541.4
  16. Mechanism of Preventing Mouse from Insulin Resistance by Chlorogenic Acid,S865.13
  17. Relationship between Polymorphism of NAT1、NAT2 and ACE Gene and Hair Dye Dermatitis,R758.2
  18. Plasma Very Long Chain Fatty Acids Levels and Its Relationship with Insulin Resistance and Hyperandrogenism in Polycystic Ovary Syndrome Women,R711.75
  19. RAS dysfunction induced by high glucose in renal tubular epithelial cells and its mechanism of action,R692.9
  20. The Investigation of the Mechanism of Reno-protective Effect on 3/4 Nephrectomized Rats with Different Protein Diets,R692

CLC: > Medicine, health > Internal Medicine > Endocrine diseases and metabolic diseases > Islet disease > Diabetes
© 2012 www.DissertationTopic.Net  Mobile