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Objective To research the effect of A3NEP1-40 (Nogo receptor antagonist) on axon regeneration and recovery of spinal cord function after spinal cord injury on the adult rat.Methods 60 healthy female SD rats were randomly divided into blank group, control group and experimental group . Rat model of acute spinal cord injury was established at the ninth thoracic vertebra level by the improved Allen weight dropping. Experimental group was injected with A3NEP1-40 (5μl,100μmol/L), the control group injected with NEP1-40 (5μl,100μmol/L) and the blank group injected with 5μl PBS buffer, and then a micro-catheter (PE-10) was implanted in subdural teratoma. The three kinds of drugs whose dose were the same as before were injected for 15 days (twice per day) by the micro-catheter which later would be washed by 5μl PBS buffer after drug injection. Postoperatively, in the 1st and 3rd day, and in the 1st, 2nd, 3rd, 6th, and 8th week, rat motor function was evaluated by BBB score. In the 1st, 2nd, 4th, and 8th week after the operation, after embedding and sectioning ,HE staining was adopted to test the pathological changes of the site of injury. And NF-200 of the lesion site was detected by immunohistochemistry.Results In the slice of the experimental group and the control group, there were more NF200 positive cells than the blank group (after 2 weeks). BBB behavior score was higher in the former two groups than the latter. And this difference has statistics meaning (P<0.05). The difference of NF200 positive cells and BBB behavior score between control group (NEP1-40 group) and experimental group (A3NEP1-40 group) was not statistically significant (P>0.05).Conclusion A3NEP1-40 was able to promote axon extension and rehabilitation of motor function on rat after spinal cord injury; it will lay a solid foundation for the next step experiment, that is, comprehensive treatment of spinal cord injury by slow-releasing of A3NEP1-40 and other factors of spinal cord repair on the scaffold material carrier.
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