Dissertation > Excellent graduate degree dissertation topics show

Induced Differentiation Suppresses the Stem Cell Porperties and Tumorigenesis of Stem-like Glioma Cells

Author: HuFeng
Tutor: LeiZuo
School: Huazhong University of Science and Technology
Course: Neurosurgery
Keywords: stem-like glioma cells ATRA differentiation stem-like porperties
CLC: R739.4
Type: Master's thesis
Year: 2011
Downloads: 4
Quote: 0
Read: Download Dissertation

Abstract


Objective Use ATRA to induce stem-like glioma cells to differentiate and compare stem-like cells properties and biologic characteristics between stem-like glioma cells, including C6 cell lines, primary glioma cells and their differentiated cells.Methods Glioma cells were cultured with neurosphere-formation assay in serum-free supplemented medium. Stem-like glioma cells (SLGC) were differentiated with all-trans retinoic acid(ATRA)-containing medium. Growth characteristics, including morphological differences, capacity of self-renewal, were detected contrastively through neurosphere formation assay. Expressions of stem cell and differentiated cell markers were determined by reverse transcription polymerase chain reaction (RT-PCR), Western Blotting and Flow Cytometry(FCM). SLGC and differentiated cells were implanted stereotactically and intracranially into the Balb/c nude mice to contrast the capability of tumor initiation and the tumor models were detected by hematoxylin and eosin (HE) staining. All data analyses were performed with the SPSS software, version 17.0.Results Our data suggest that in vitro differentiation of SLGCs with ATRA induces the growth characteristics changes of glioma cells ,down-regulation of CD133 and Nestin expression and up-regulation of GFAP. In vivo mouse model, differentiated cells inoculated mice have better survival outcome. Conclusions1.ATRA can induce the differentiation of SLGCs.2.The tumorigenicity of stem-like glioma cells is reduced after ATRA differentiation.3.Differentiation treatment (ATRA) targeted the stem-like cell population is a candidate for adjuvant therapy against malignant gliomas.

Related Dissertations

  1. Exoression of Resistin During Porcine Preadipocyte Differentiation and Its Infiuencing Factors,R587.1
  2. Professor Wang Qi identified the body - of Diseases - dialectical combination of academic thought and clinical experience and treatment of chronic insomnia clinical studies,R249.2
  3. miRNA-23a into the regulation of muscle differentiation in C2C12 cells,Q952.5
  4. miR - 23 in mouse skeletal muscle expression and the regulation of skeletal muscle regeneration research,Q78
  5. Evaluation of Land Use Eco-security in Fujian Province,X826
  6. Variations of Host-Specialized and Migratory Biotypes of Cotton Aphid on the Mating Behavior and Genes Sequences of Mitochondrion DNA and Symbiotic Bacteria,S433
  7. Genetic Characterization and Differentiation of Natural Populations of Wild Soybean from Middle and Lower Valleys of Huanghe and Changjiang as Well as Their Genetic Relationship with the Cultivated Soybean,S565.1
  8. The Molecular Systematics and Classification of Triticeae Polyploids with St Genome,S512.1
  9. Effects of Isoprocarb Exposure on Differentiation, Migration and Neurite Outgrowth of the Key Cell During Neural Development in Vitro,R329
  10. The Study of Rabbit Bone Marrow Mesenchymal Stem Cells Separation Culture and Directional Differentiation,R329
  11. University Press Digital Publishing and Countermeasures,G237.6
  12. Genetic Diversity, Population Sturucture and Gene Flow of Helicoverpa Armigera (H(?)bner) in China,S435.622.3
  13. Identification of Biological Characteristics with Human Keloid Fibroblast-like Mesenchymal Stem Cells,R622
  14. Study on the Physiological Differentiation and Genetic Diversity of Exserohilum Turcicum in Northeastern China,S435.131
  15. Study on Genetic Diversity on ORFs Regions and Indica-Japonica Consanguinity Differentiation of Weedy Rice,S511
  16. Optimization Study on BHF Marketing Strategy,F274
  17. The Comparative Study About Some Growth Indexes and Spike Differentiation of Wheat Under Greenhouse and Field,S512.1
  18. Molecular Cloning of CYP19 Gene and Their Expression of Adult and Embryo in Two Species of Loaches,S917.4
  19. Clinical Study on Relationship Among Syndrome Differentiation Clssified in Tcm,ambulatory Blood Pressure Monitoring and Transcranial Doppler in Essential Hypertension,R259
  20. Psoriasis vulgaris in different stages and card Hou T lymphocyte subsets,R275.9
  21. Effects of Ginsenoside Rg1 on Cell Proliferation, Secretion Function and ERK、p38 Signal Molecular in Human Umbilical Cord Blood Mesenchymal Stem Cells under Optimized Culture Condition,R285.5

CLC: > Medicine, health > Oncology > Nervous system tumors
© 2012 www.DissertationTopic.Net  Mobile