|
Objective To study the TGFβ on cell proliferation in human lung adenocarcinoma cell line A549 cell invasion , and to investigate the mechanism they produce , on this basis, to reveal the relationship between TGFβ and the degree of malignancy of lung cancer cells , gene therapy for lung cancer provide a new target and experimental basis . The Methods siTGFβR2 transfected and TGFβ1 stimulate cells to inhibit and promote the biological effects of TGFβ signaling MTT assay cell proliferation rate , flow cytometry to detect apoptosis , western blot detection of protein expression , cell scratch hair the detection cell migration , Transwell chamber the detection cell invasion ability. Results 1. TGFβ1 can promote the proliferation of A549 , inhibition of apoptosis ; the Add siTGFβR1 can be suppressed cell proliferation . 2. TGFβ can promote the expression of PI3K . 3. TGFβ can promote the invasion and metastasis of A549 promote cell MMP -2 , MMP - 9 expression , inhibition of the expression of E-Cadherin . 4. TGFβ can promote the expression of Erk1 / 2 , and the above-mentioned effects can be reversed blocking Erk1 / 2 signal . The conclusion TGFβ can promote the proliferation and invasiveness of the A549 , to enhance the degree of malignancy of cells , TGFβ may activate the PI3K/AKT promoting proliferation and promote the invasion may Erk1 / 2 mediated matrix metalloproteinase overexpression and E -Cadherin low expression .
|