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This topic is silver nitrate as the precursor , the use of sodium borohydride and sodium citrate two reductant simultaneously, as a first step a reducing agent sodium borohydride , in the coexistence system of sodium borohydride and sodium citrate in reducing nitrate silver , starch as a protective agent , made ??under the action of sodium citrate with a uniform particle size of the nano - particles of the product under the conditions of each of the reagents added in an amount changed ultraviolet - visible absorption spectra of the reaction process . Then as a test strain of Escherichia coli and Staphylococcus aureus were tested antibacterial, bactericidal properties of silver nanoparticles under different conditions , including temperature, time, concentration , particle size , the use of transmission electron microscopy on the silver nanoparticles during sterilization follow-up observations , from the micro-level sterilization mechanism of action . The experimental results showed that the silver nano- particles in 400 ul of 0.2 mmol ยท L -1 was added to the homemade 1 mL of E. coli solution ( 600 / ml ) , it can have a significant bactericidal effect . Increase in the concentration of silver nanoparticles , the sterilization time , the sterilizing effect is significantly enhanced ; temperature have a significant effect on the bactericidal effect , after the temperature reached 37 degrees Celsius , the significant bactericidal effect , bactericidal effect is reduced below this temperature , the silver nanoparticles ; silver nanoparticles has a killing activity against a variety of bacteria , especially Staphylococcus aureus bactericidal effect is better . Silver nanoparticles smaller , the better the effect . Without reduction of Ag bactericidal effect compared with the same concentration of silver nanoparticles , which can be judged of Ag and the silver nanoparticles mechanism should there is a large difference . Silver nanoparticles with hydrogen peroxide , cetyl trimethyl ammonium bromide compound results showed that silver nanoparticles in two different reagents under the action of the bactericidal effect is more prominent .
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