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Capillary electrophoresis (CE) and high performance liquid chromatography (HPLC) is the extremely wide range of modern separation techniques in two applications, they are highly efficient, fast, high degree of automation and sample consumption less time in ten years years, especially in recent years, been of great concern for the separation and analysis of scientists, has become the most watched in biochemistry and analytical chemistry, the fastest growing two separate analysis of new technologies. Nonaqueous capillary electrophoresis (NACE) is a branch of capillary electrophoresis, not only has the characteristics of the general pattern, and has its own unique characteristics and advantages. Expanding the scope of its research, including inorganic ions, neutral substances, organic acids, peptides, drugs separation, covering both basic research and analysis applications. However, many non-aqueous solution of physical and chemical properties are not very clear, and a wide variety of organic reagents, this reagent gave the choice to bring certain difficulties. The solvent system selection rules and optimization methods should be the focus for the next time, and provides researchers with a lot of issues. Amino acids and small peptides is the most basic life activities related substances, has played an irreplaceable role in the in vivo metabolism and physiological processes. Capillary electrophoresis separation of amino acids and small peptides, has a very important significance in the field of medicine, food, health. But most of the amino acids and peptides is not only weak UV absorption, direct UV detection to meet the requirements can not determination of low levels of compounds. Widely used derivative reagent 9 - fluorenyl methoxycarbonyl chloride (FMOC), o-phthalaldehyde (OPA) and 5 - dimethylamino naphthalene-1 - sulfonyl chloride (DNS-Cl), but these derivative reagent some characteristics determine the limitations of its application. Aromatic amines toxicity is relatively high, mainly enter the body through the skin, respiratory and digestive tract, its toxicity and potential carcinogenicity brought a huge risk to people's daily lives. Therefore, the analysis and detection of aromatic amines is very necessary, must be to establish a simple, rapid, highly sensitive method of measuring. Laboratory synthesis of novel derivatives reagents derivatization of amino acids, small peptides and aromatic amine derivatives of amino acids and small peptides, respectively, then the non-aqueous capillary electrophoresis separation technology using high-performance liquid chromatography of aromatic Derivatives of the separation and analysis. The thesis is divided into four chapters: the first chapter: a brief introduction to the development of capillary electrophoresis in drug analysis, biomolecule separation, the application of genetic engineering research, clinical medicine, and non-aqueous capillary electrophoretic separation of analyte mode its basics elaborate. Chapter II: non-aqueous capillary electrophoresis on a variety of amino acid derivatives were separated. First, using a new synthesis of UV-derived reagents 10 - ethyl-acridine-one-2 - sulfonyl chloride on a mixture of amino acids derivatives, then the mode of non-aqueous capillary electrophoresis separation of a variety of derivatives, and discuss the need to optimize the separation and determination of the multi- argument, the experimental results are satisfactory. Chapter: 10 - ethyl of Acridine-2 - chloride as pre-column derivatization reagent nonaqueous capillary electrophoresis to examine and optimize the conditions of the separation of a variety of small peptides. Plus other additives can be realized five small peptides efficient baseline separation, under optimal conditions, the samples were measured, the results were satisfactory. Chapter IV: a brief introduction of the principle of chromatography and classification, focuses on the development and practical application of high performance liquid chromatography, and dye aromatic amine derivatives were separated by high performance liquid chromatography with mass spectrometry. The fluorescent labeling reagent 2 - [2 - (7H-dibenzo and [a, g] carbazole)] - isopropyl chloride carboxylate (DBCPC-Cl) aryl amine compound as a pre-column derivatization reagent for efficient liquid chromatography - mass spectrometry detection, optimized by derivatization and HPLC conditions established aromatic amines column derivatization high-performance liquid chromatography with fluorescence detection, and the method is applied to the determination of the relevant real samples, results indicate that the developed method has great feasibility.
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