Dissertation > Excellent graduate degree dissertation topics show

The Effects of NO Content and NOS Activity of NSKKFY Ⅱ on Myocardial Ischemia in Rat

Author: LiXue
Tutor: DongYuXiang
School: Jilin University
Course: Traditional Chinese Medicine
Keywords: NXKKFYⅡ myocardial ischemia(MI) NO NOS
CLC: R285.5
Type: Master's thesis
Year: 2011
Downloads: 63
Quote: 0
Read: Download Dissertation

Abstract

Clinical manifestation of myocardial ischemia is a pathological state mainly due to vascular endothelial injury and blood vessel’s lipidosis which produce a parade of reactions leading to vascular stenosis. Its main inducement is coronary atherosclerosis.It’s to be of the opinion that the injury mechanism is related to oxygen free radical, energy metabolism disorders, intracellular calcium overload and PMN invasion. CAHD is short for coronary atherosclerosis heart disease caused by ischemina of vessel occlusion. As a high incident disease in our country, it has severe effect in people’s life and endangers patients’ life. Myocardial ischemia resulting in cardiac myocyte ischemia will bring the consequence of cardiac myocyte’s disordered electronphysiology and change of cellular form and function which at last causes cellular death. So it’s an extreme urgent to Prevent it at an early stage. The Chinese Medicine Department of Jilin University First Hospital has been engaged in clinical prevention and research in CAHD. In order to effectively prevent and cure CHAD, improving the blood supply to patients’coronary artery, our department, based on Chinese medical theory, choose medicine promoting blood circulation by removing blood stasis to confect NXKKFYⅡ. This medicine is mainly applied to prevent and cure CHAD caused by obstruction of meridian by blood stasis and phlegm. According to its conception of composition and clinical research in various medicines, this prescription is supposed to effectively suspend the occurring and expanding of CHAD, furthermore, it can obviously improve the patients’myocardial ischemia and release their discomfort. This experiment is with a goal to discuss the effect of NO.2Naoxinkang oral liquid on the NO and NOS in blood serum by setting up animal model as well as its protection and related mechanism of action. Thus it can provide experimental basis of clinical medicine use.In this study, the rats are randomly divided into 6 groups,they are normal group, the group of model of MI, the group of DXSYSLZJN, and the group of NXKKFYⅡof low-dose, midst-dose and high dose,and there are 10 rats in each group. After 20 days’ treatment of the medicine, we inject high dose PIT continuous to the rats in celiac and establish myocardial ischemia (MI) model, and then, for ECG monitor after inject PIT twice. making sure the model is right, we anesthetize the rats, take and centrifugal abdominal aortic blood, collect the supernatant. Then testing the changing of the content of NO, activity of NOS in the blood-serum. Get the myocardial tissue and observe myocardial injury Finally. The result of the experiment is the 90% rats in the model group show the appearance of myocardial ischemia, compared with normal group the difference is significant (P<0.01),made model successful. But the positive rate of myocardial ischemia of groups of NXKKFYⅡeach dose was significantly lower.and better than the group of model of MI. It shows that each group of NXKKFYⅡcould improve the abnormal ECG effectively. high-dose group the most significant difference (P<0.05),and there no significant difference with the group of DSDW (P>0.05). Histopathological observation showed that each dose group of NXKKFYⅡcould protect and improve Myocardial cells in ischemia condition and the high-dose group is most obvious, its cell form close to normal. This study demonstrate that NXKKFYⅡcontains the role of protection to myocardial ischemia injury by the affect to Endothelial active substances, myocardial cell electric physiology and myocardial histopathological.NO catalytic generated by NOS. The direct role of endothelial cell protection of NO is hemangiectasis, and it also could restrain many blood ingredients adherent to the vascular endothelial cells and fight blood-vessel walls cell proliferation. NO could antioxidant and inhibit gathering、adhesion and activation in ischemia area of, PMN and PLT, and clear the oxygen free radical from PMN. From the three aspects above, we can see, NO could protect myocardial ischemic injury effectly. This experiment finds that NOS activity declined and NO content reduced in myocardial ischemia process, and each dose group of NXKKFYⅡwere can improve NOS activity and NO content, and the high dose group and midst-dose group obviously (P<0.05), when it compare with the group of DSDW,there is not obvious significant difference (P>0.05). therefore, NXKKFYⅡcould protect myocardial by increasing the activity of NOS and the content of NO.Chinese traditional medicine define CHAD as the blood stagnancy and deficiency of QI. lead to the ischemic and hypoxia myocardial disease together. Then principal of cure is to supply the QI and promoting blood flow. NXKKFYⅡselect the drug of promoting blood circulation and Phlegm dredging. All of them could protect the myocardial ischemia injury.To sum up, we can get the following conclusion from this study is that the NXKKFYⅡcan effectively improve the abnormal ECG, stable myocardial cell electrophysiology; And NXKKFYⅡprotect the form and structure of myocardial.These aspects confirmed that NXKKFY II the role of clear protection in myocardial ischemia injury rats.Mechanism is that NXKKFY II could improve the activity of NOS and increase of myocardial protection effect of NO.

Related Dissertations

CLC: > Medicine, health > Chinese Medicine > Of Pharmacy > Pharmacology > Chinese medicine Experimental Pharmacology
© 2012 www.DissertationTopic.Net Mobile