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Studies on the Extracting Technique of Total Flavonoids from Tartary Buckwheat Bran

Author: GuTao
Tutor: WangYanHan
School: Southwestern University
Course: Microbial and Biochemical Pharmacy
Keywords: buckwheat bran total flavonoids extract purification technique
CLC: R284.1
Type: Master's thesis
Year: 2011
Downloads: 129
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Abstract


Tartary buckwheat bran is rich in flavonoids which present universal pharmacological and physilogical activities such as anti-free radical, against binding of platelet activating factor, protecting from Acute Cerebral Ischemic Injury and improving the disorder of glucose and lipid metabolism.This work dedicated to optimizing the extracting technique for the total flavonoids extract from Tartary buckwheat bran (TBBTFE). Firstly, quantitative determination method of total flavonoids was established with spectrophotometry method by coloration with AI5+ and using rutin as reference substance. Proper amount of sample solutions was added with 1 mL of AlCl3·6H2O-ethanol solution, then with 70% ethanol solution to the total volume to be 10 mL, incubated for 4h at room temperature and the Absorbance was determined at 410 nm. The effects of the technological factors including extracting method, the concentration of ethanol aqueous solution, the ratio of solvent to stuff and extracting time on the total flavonoids determination were investigated by the single-factor research and orthogonal design. Finally, the preparation method for sample solution was determined as follows:extracting the material with the help of ultra sound for 30 min with 70% aqueous ethanol as solvent, with the ratio of solvent to stuff 1:50.Next, the extracting technique for TBBTFE was optimized. The extracting rate of total flavonoids was used as the index to assess the extracting technological conditions. Ultrasonic extraction, reflux and percolation were investigated. Finally, the extraction processes for TBBTFE were determined as follows:extracting the material by reflux with 10 times of 70% ethanol aqueous solution for 2h and extracting for twice.Last, the purification technique for total flavonoids from Tartary buckwheat bran was optimized with different macroporous adsorbent resin. ML-7、HP-20、ML-40、HD2MGL、HP-20SS、D202、FPDA、CD180、122、717、711、CM650、D004、D151 and 731 were investigated for purification of total flavonoids. ML-7、HP-20 and ML-40 exhibited better properties of adsorption and desorption for total flavonoids than other resins in static state. After comparation the loading amount and desorption rate of the three in dynamic state, ML-7 showed to be the most sutiable macroporous adsorption resin for purification of total flavonoids from Tartary buckwheat bran. The eluent was determined as 70% ethanol solution and the elution volume is 4BV by the curve of accumulation desorption rate.

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CLC: > Medicine, health > Chinese Medicine > Of Pharmacy > Traditional Chinese medicine chemical > Chemical analysis and identification
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