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This thesis is divided into three parts the first part of the literature review in this section inspection in the system in 2010 on the basis of the relevant literature, cycad fern rhizome and Ukraine Cyclosorus kossou iron fern genus, genus Blechnum, Woodwardia the case of plants origin, chemical composition and pharmacological studies summarized progress, the formation of the Summary. Experimental study of the second part of the cycad fern rhizome. Cycad fern rhizome chemical composition studies after repeated silica gel column chromatography, spectroscopy (UV, 1H-NMR, 13C-NMR, 2D-NMR) and chromatographic means (TLC ), with reference to the relevant literature, eventually identified eight compounds isolated from the roots of the cycad fern (Brainea insignis (Hook.) J.Sm), were protocatechuic acid, 4 - (3,4 - hydroxyphenyl ) -3 - buten - 2 - ketone, p-hydroxybenzoic acid, pods Fern prime-7-O-glucoside, Amentotaxus Biflavones ,5-O-caffeoyl - shikimate slope that steroid the ketones-A and the slope That steroidal glycoside-A, where the top five for the first time isolated from this plant. Another two compounds structure has not been determined, the one based on the hydrogen spectrum initially judged as sterols. Cycad fern rhizome protocatechuate and cycad fern rhizome protocatechuate and parabens HPLC determination method hydroxybenzoic acid assay method of research based on methodological study, cycad fern rhizome of different origin Determination of two components, the results show that: protocatechuic in the injection volume 0.0202μg ~ 0.1414μg within the peak area showed a good linear relationship, the regression equation: Y = 4143.2X-3.275 (r = 0.9999), hydroxybenzoic acid in the injection volume was 0.006 μg to 0.042μg range and peak area showed a good linear relationship, the regression equation was Y = 5353.2X-0.6667 (r = 0.9999) . Precision, stability, repeatability and processing recoveries are in line with the requirements. In addition, through the comparison can be found also there are some differences in the content of the two components in eight batches of six different origin cycad fern rhizome, which the Hainan Haikou production the cycad fern Guanzhong protocatechuic highest content (0.1314mg / g) Nanjing was the lowest (0.0752 mg / g); hydroxy benzoic acid, Haikou production cycad fern the Guanzhong highest (0.0535 mg / g), the Guangzhou lowest (0.0177 mg / g). 3 different origin cycad fern rhizome the slope that ketosteroid-A and slope steroidal glycoside-A Determination Our previous HPLC method for simultaneous determination of different origin cycad fern rhizome middle ketosteroid-A The results show that: and slope that steroidal glycoside-A content can be found by comparing the content of the two components there are some differences in the eight batches of six different origin of the cycad fern rhizome, Guangdong Guangzhou production cycad fern Guanzhong slope that steroidal glycoside-A highest content (4.4729mg / g), was the lowest in Longhai, Fujian (2.4826mg / g); Nanjing production cycad fern rhizome slope ketosteroid-A content the highest (0.2642mg / g), Yunnan Kunming lowest content (0.1057 mg / g), of other origin cycad fern rhizome slope steroidal glycosides-A and slope ketosteroid-A content in this range. 4 cycad fern Guanzhong Study on the Determination of Total Flavonoids hydrochloric acid - the magnesium powder colorimetric established cycad fern rhizome method for the determination of total flavonoids. Methodological study based on the content determination of total flavonoids of different origin cycad fern rhizome. The results show that: the reference substance rutin in concentration 0.0115mg/ml-0.1261mg/ml within and absorbance was good linear relationship, the regression equation was Y = 6.5952X-0.0299 (r = 0.9997), precision, repeatability, stability and recovery of the experimental results are in line with the requirements. In addition, through the comparison can be found consistent total flavonoids in eight batches of six different origin of the cycad fern rhizome (RSD = 4.19%), from Kunming, Yunnan cycad fern rhizome total flavonoids highest (12.24%), Jiangsu Nanjing production was the lowest (10.91%), the other cycad fern origin Guanzhong total flavonoids are within this range. Third section summarizes the discussion of the papers by the chemical composition and quality of the cycad fern rhizome draw the following conclusions: First, the cycad fern the Guanzhong contained the chemical composition class is very complex, including the steroid, flavonoids (flavonoids, flavanone flavonoids), phenylpropanoids class and small molecule organic acids. From a content point of view, steroid, flavonoids and small molecular organic acids as the main ingredient, which slope steroidal glycoside-A content up to 4.4729mg / g (Guangzhou), the protocatechuic content highest 0.1314mg / g (Haikou); the single flavonoids are not high, but many of its kind, the total flavonoid content in the range of 10.91% -12.34%. Secondly, from the viewpoint of the pharmacological activity, the main component of the cycad fern rhizome protocatechuate having antibacterial activity and parabens, slope that steroidal ketone-A and the slope that steroidal glycoside-A have anthelmintic Flavonoids have antioxidant, antibacterial and anti-tumor effect, these pharmacological activities are consistent with the Pharmacopoeia cotton horse Guanzhong cycad fern in clinical the Guanzhong use provides a theoretical basis for the further development and utilization cycads fern rhizome laid the foundation.
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