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Chinese Magnolia active ingredients fluorescence analysis of

Author: YaoJie
Tutor: WeiYongJu
School: Hebei Normal
Course: Analytical Chemistry
Keywords: Magnolia Magnolol Honokiol Ionization Constants Fluorescence quantum yield Fluorescence Analysis TLC scanning absorbance Three-dimensional fluorescence spectra
CLC: R284
Type: Master's thesis
Year: 2011
Downloads: 79
Quote: 0
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Abstract


Studied medicine Magnolia (Officinal Magnolia Bark) active ingredient Magnolol and Magnolol fluorescence spectra of experimental conditions investigated the influence on the fluorescence spectra , fluorescence properties based on the difference between the two is established without separation and determination of thickness Park herbs Magnolol and Magnolol content solution fluorescence method . Magnolia also studied medicine three-dimensional fluorescence fingerprints , herbs can be used for qualitative identification of Magnolia . Thesis work is divided into four parts: a study of the active ingredient in traditional Chinese medicine Magnolia magnolol and honokiol fluorescence spectroscopic properties . Acidity were investigated , surfactants and solvents and other experimental conditions on the fluorescence properties of , with the pH- absorbance measured by Magnolol ionization constant pK_a = 7.67, with a pH- fluorescence measured ionization constant pK_a = Magnolol 7.54 . To L-tryptophan as a reference, in the near-neutral conditions, Magnolol measured at the maximum at 286 nm excitation wavelength of the fluorescence quantum yield of 0.36 , and the maximum excitation wavelength Magnolol fluorescence at 258 nm quantum yield of 0.31 . 2 . studied medicine officinalis extract fluorescence spectra , according to Magnolia, Magnolol and spectral properties Magnolol established simultaneously without separation DETERMINATION Magnolia Magnolol and thick Park phenolic content of the solution fluorescence method , the measured sample Magnolia Magnolia phenol content was 0.97% , and 0.75% honokiol content . 3 for the determination of Magnolia Magnolol and honokiol TLC , the measured sample Magnolia magnolol and honokiol contents were 0.98% and 0.77% , consistent with the solution fluorescence method . 4 Magnolia studied medicine three-dimensional fluorescence fingerprints examined the reproducibility of the fluorescence spectra , analytical methods durability and fingerprint specificity , established the optimum conditions for the analysis , which was established using three-dimensional fluorescence fingerprints on the qualitative and quantitative analysis of Magnolia new method .

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CLC: > Medicine, health > Chinese Medicine > Of Pharmacy > Traditional Chinese medicine chemical
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