|
Objective: To observe moxibustion Neiguan, ischemia-reperfusion injury rabbit serum nitric oxide (ni-TRIC oxide, NO), nitric oxide synthase (nitric oxide synthase, NOS) content gap detection of myocardial mitochondrial connection changes in expression levels of proteins (Connexin43, Cx43) explore moxibustion rabbits \the moxibustion Neiguan prevention of myocardial ischemia provide the basis. : 32 New Zealand white rabbits were randomly divided into four groups, each of eight. Model group, EA Neiguan group, Neiguan group moxibustion, sham-operated group. Ligation rabbit coronary left anterior descending branch of myocardial ischemia and reperfusion injury model, after the end of the experiment, nitrate reductase method for measuring serum NO, NOS in immunohistochemistry SP method in the detection of myocardial cells Cx43 expression levels. Results: Serum NO, NOS in the model group compared to sham group (P lt; 0.05); Electroacupuncture of Neiguan serum NO, NOS in higher than the model group (P lt; 0.01) and sham group (P lt; 0.05), the moxibustion neiguan serum NO, NOS is also higher than the model group (P lt; 0.01) and sham group (P lt; 0.05), moxibustion Neiguan group EA shut serum NO the difference was not statistically significant, NOS in 2. the Cx43 expression 1OD by the model group rabbit cardiomyocytes mean significantly lower than those in the sham surgery group (P lt; 0.01) Electroacupuncture of Neiguan group, moxibustion Neiguan group with the sham group center muscle cells Cx43 expression 1OD mean difference was not statistically significant; myocardial cells Cx43 Electroacupuncture of Neiguan group the expression of 1OD mean is higher than the model group (P lt; 0.01), moxibustion Neiguan group myocardial cells the 1OD Cx43 expression was also higher than the mean model group (P lt; 0.01), Neiguan group moxibustion Neiguan group EA myocardial cells Cx43 expression 1OD mean difference was statistically significant conclusions: 1. moxibustion Neiguan may increase serum NO, NOS is activated endogenous protective substances, increased myocardial gap protein Cx43 involved in myocardial electrical coupling and metabolic coupling, to protect ischemia-reperfusion myocardial cells. Neiguan EA. Moxibustion Neiguan compared improve myocardial ischemia and reperfusion in rabbit serum NO NOS content, and the gap protein Cx43 expression in myocardial cells, the difference was not statistically significant. Tip Neiguan EA Neiguan moxibustion are likely through the activation of endogenous protective substances and increase in cardiac gap junction protein expression, protection of ischemia-reperfusion myocardial cells. Moxibustion its method is simple, safe, patients feel comfortable and easy to accept, it is worth to promote the prevention of clinical myocardial ischemia.
|