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Comparative Study on Developmental Toxicity in Vitro Mouse Whole Embryo by Three Kinds Nano- and Micro-scale Materials

Author: DingShuJuan
Tutor: ZhangTianBao
School: Second Military Medical University
Course: Health Toxicology
Keywords: Nano-oxide Whole embryo culture In vitro Embryotoxicity
CLC: R114
Type: Master's thesis
Year: 2011
Downloads: 54
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Abstract


The nano material security is the current toxicological new hot research field. Developmental toxicity is one of the important content of toxicological safety assessment. What is the difference cytotoxicity characterized the development of nanomaterials with the same chemical composition compared to the conventional scale materials, whether the the current developmental toxicology security evaluation method for developmental toxicity evaluation of nanomaterials is the immediate question. In this study, three different scales nano-oxide (Nano-TiO 2 Nano-SiO 2 Nano-ZnO) conventional scale materials with the same chemical composition as the subject test material, through a comparative study differentiation from embryonic growth and development as well as tissue and organ morphology perspective reveals embryonic developmental toxicity and characteristics of the nano-oxides, the purpose is to provide the basis for the accumulation of data for the correct evaluation of the developmental toxicity of nanomaterials. Methods: ICR mice pregnant 8.5d off by cervical dislocation, separation embryos, using whole embryo culture (whole embryo culture, WEC) methods, respectively: (1) three kinds of nano-scale Nano-TiO 2 (5 10nm, 60nm, 90nm) and the conventional scales TiO 2 , 0.0, 50.0, 100.0, comparison of the impact of the growth and development of the murine embryonic the 200.0μg/ml dose; ② four kinds of nano-scale Nano -SiO 2 (15nm, 30nm, 50nm, 100nm) and conventional scale SiO 2 , 0.0, 50.0, 100.0, 200.0μg/ml doses on murine embryonic Comparison of growth and development; ③ three kinds of nano-scale Nano-ZnO (30nm, 50nm, 100nm) and conventional scale ZnO in 0.0, 25.0, 50.0 Comparison of the effects of the growth and development of the murine embryonic, 100.0μg/ml doses. The results: 1. Conventional scale TiO 2 was not observed under the conditions of this experiment to the developmental toxicity; TiO 2 the dose reach 100--200.0μg/ml can be microscope to the yolk sac surface nano-particles scattered in distribution, while conventional TiO 2 was not observed this phenomenon. Three different scales Nano-TiO 2 mouse embryos with embryo toxicity and teratogenicity, the performance of the yolk sac and embryo growth retardation, tissues and organs morphological differentiation score decreased abnormal embryo morphology (nerve dysraphism incomplete posture flip, somites disorders did not develop in the back of the head, hydrocephalus, hindlimb buds) sensitivity from the developmental point of view, the most sensitive to the morphological differentiation of the nervous system in 50.0μg / ml can be affected. As the dose increased, the affected organs and tissues increased, the severity of the impact on development in 100.0μg/ml over dose can lead to abnormal shape of the mouse embryo, in 200.0μg/ml doses can also cause death of the embryo. Different time results the 200.0μg/ml doses heavier with increasing duration of action, the impact of the growth and development of the embryo. The of different scales nano TiO 2 different growth and development of the embryo, the descending order of severity embryonic developmental toxicity of 5-10nmTiO 2 gt; 60nmTiO 2 gt; 90nmTiO 2 . 2. Conventional scale SiO 2 obvious developmental toxicity was not observed. Different nano-scale SiO 2 in above in 100.0μg/ml dose can cause murine yolk sac diameter, cranial rump length, head length and number of somites indicators morphological differentiation and tissue and organ indicators scores decreased, and 50nmSiO 2 100μg/ml dose group and 15,30,100 nmTiO, 2 in 200.0μg/ml dose group, abnormal embryo morphology The organ; dose reached 200.0μg/ml 50 nm SiO 2 individual embryos can lead to death. With the increase in the duration of action of the same scale with the increasing dose or the same dose, the affected organs and tissues increased severity of the impact on development. The different scales Nano-SiO 2 50 nm the SiO 2 relative to 15,30,100 nm the SiO 2 serious damage growth and development of the embryo, and With increasing doses performance more obvious; 100 nm SiO 2 toxicity relative minimum. Conventional ZnO is not observed significant developmental toxicity. Three different scales of nano-ZnO above in 50.0μg/ml dose could lead to the growth and development of tissues and organs of mouse yolk sac diameter, cranial rump length, head length, and somite number morphological differentiation the indicator score lower and in 30,50 nmZnO in the dose group and 100nmZnO of 50.0μg/ml more teratogenic and lethal in 100.0μg/ml dose group. With the increase in the duration of action of the same scale with the increasing dose or the same dose, the affected organs and tissues increased severity of the impact on development. 30nmZnO 50,100 nm ZnO in lower doses (25.0μg/ml), on embryonic growth and development can be observed damage and with increasing doses 30nmZnO on the in vitro mouse embryonic growth and development compared to the other two dimensions more The serious, embryo mortality was significantly higher than the other two scales, the descending order of severity of the three scales of nano-ZnO organ differentiation and embryonic mortality 30nmZnO gt; 50nmZnO gt; 100nmZnO. Conclusion: The three kinds of nano-and conventional-scale materials there are obvious differences in the nature of the development of mouse embryos in vitro cytotoxicity. Nano-TiO 2 , Nano-SiO 2 and Nano-ZnO ICR mice embryos have obvious developmental toxicity, three conventional scale materials in the same dose under no obvious developmental toxicity. Three kinds of nano-oxide (Nano-TiO 2 Nano-SiO 2 Nano-ZnO) several different scale materials on mouse embryos in vitro developmental toxicity characterized by three types of nano-scale materials have embryo toxicity and teratogenicity; were, by affecting tissues and organs increased with increasing dose and exposure time, the impact severity and even death of the embryo, showing a significant dose - response relationship and time - - effect relationship; three types of nano-scale materials have a wide range of tissues and organs, not observed apparent specific target organ; affect the dose of embryonic development of the yolk sac development also have an impact; three nanomaterials Nano-ZnO greater toxicity, 30nano-ZnO 25.0μg/ml 50 100nano-ZnO 50.0μg/ml have significant developmental toxicity, and the the different scales Nano-TiO 2 Nano-SiO 2 to render developmental toxicity 100.0μg/ml over dose. 3 different size of different scale nano-oxides on mouse embryonic development. Nano-TiO 2 and Nano-ZnO nano scale smaller on cultured mouse embryonic growth and development and the impact of tissue and organ differentiation aggravated obvious scale effect. Nano-SiO 2 obvious scale effect 15,30,50,100 nm to 50 nm SiO 2 toxicity.

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