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Study on Nutritional Evaluation of Feed for Ruminants by in Vitro

Author: HaoJianXiang
Tutor: ZhuWeiYun
School: Nanjing Agricultural College
Course: Agricultural Extension
Keywords: Roughages Meals and cakes Combination In vitro fermentation Nutritive value
CLC: S816
Type: Master's thesis
Year: 2011
Downloads: 48
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Abstract


The nutritive value of ten roughages and five meals and cakes were evaluated by in vitro The fermentation characteristics of ten roughages and five meals and cakes were studied in vitro and the nutritive value of the combination of selected roughages and the same concentrate were also evaluated by in vitro in this study. This thesis was described in the following three sections.1 Study on nutritional evaluation of roughages by in vitroThis experiment was aimed to evaluate nutritive value of different roughages in vitro. The rumen contents from three local male goats with permanent rumen fistula were used as inoculum. Ten roughages were used as substrates including wheat husk, Chinese wildrye hay, rice straw, high-energy forage, Chinese alfalfa, American alfalfa, whole-plant corn silage, peanut vine silage, straw silage corn, and hybrid sorghum silage. The fermentation was stopped at 48 h. Straw silage corn and whole-plant corn silage showed the highest cumulative gas volume at the end of fermentation. The pH value of the cultures varied from 6.60 to 6.79 and the culture with American alfalfa as substrate showed the highest pH value. At 48 h, the cultures with straw silage corn and whole-plant corn silage showed the highest dry matter digestibility, total volatile fatty acids, propionate and butyrate (P<0.05), and the lowest ratio of acetate to propionate (P<0.05). The culture with straw silage corn showed the highest neutral detergent fiber digestibility (P<0.05). Cultures with peanut vine silage and straw silage corn showed the highest acid detergent fiber digestibility (P< 0.05). Cultures with different roughages showed different neutral detergent fiber digestibility and acid detergent fiber digestibility (P<0.05). These results suggest that Chinese alfalfa, American alfalfa, whole-plant corn silage and straw silage corn are high potential roughages for ruminant feeds.2 Study on nutritional evaluation of meals and cakes by in vitroThis experiment was aimed to evaluate nutritive value of different meals and cakes in vitro. The rumen contents from three local male goats with permanent rumen fistula were used as inoculum. Five species of meals and cakes were used as substrates including soybean meal, cottonseed meal, rapeseed meal, sesame meal and palm meal. The fermentation was stopped at 12 h,24 h and 48 h. results showed that soybean meal showed the highest cumulative gas volume at 48 h. the cultures with soybean meal showed the highest DMD and organic matter digestibility (OMD) at 12 h,24 h and 48 h (P<0.05), at 48 h, more than 80% was completed, while others’ only 40%. At 12 h,24 h and 48 h, the microbial crude protein (MCP)concentration in the cuture with soybean was also obviously higher than other groups (P<0.05), while the sesame meal was almost the same during fermentation period. The pH value of the cultures varied from 6.57 to 6.77. The ammonia nitrogen (NH3-N) concentration in the culture with Soybean meal showed the highest at 12 h and 24 h, which was significantly higher than that of other groups (P<0.05). At 48 h, NH3-N concentration in the cultures with rapeseed meal and soybean meal were significantly higher than other groups (P<0.05). These results suggest that soybean meal is high potential protein feed sources for ruminant feeds.3 Study on nutritional evaluation of the combination of selected roughages and the same concentrate by in vitroThis experiment was aimed to evaluate nutritive value of the combination of selected roughages and the same concentrate by in vitro. The rumen contents from three local male goats with permanent rumen fistula were used as inoculum. The substrates are compound forage mixture formulated by concentrated feed and roughages mixture with the ratio 40:60. The concentrated feeds are mixture formulated by Corn, soybean meal and wheat bran with the ratio 6:3:1, the roughages mixture are straw silage corn mixed with rice straw (group 1), American alfalfa (group 2), Chinese wildrye hay, (group 3), wheat husk (group 4) with the ratio 1:1. The fermentation was stopped at 12 h,24 h and 48 h. results showed that, group 2 showed the highest cumulative gas volume at 48 h. At 12 h,24 h and 48 h, the cultures with group 2 showed the highest DMD and OMD (P< 0.05). At 48 h, DMD, OMD of group 1 and 2 arrived at 60%, which were also obviously higher than that of group 3 and 4 (P< 0.05). At 24 h, the microbial crude protein (MCP) concentration in the cuture with group 2 was highest, which was significantly higher than group 3 (P< 0.05), at 12 and 48 h, there was no significant difference among all the groups (P> 0.05). At 24 h and 48 h, the ammonia nitrogen (NH3-N) concentration in the culture with group 2 broth was significantly higher than other groups (P< 0.05). At 12 h, acetate concentration in the culture with group 1 and 2 were obviously higher than that of group 3 and 4 (P< 0.05), acetate and TVFA concentration at 24 h and 48 h after incubation were not significantly different (P> 0.05). TVFA concentration in the culture with group 2 at 12 h was significantly higher than group 3 (P< 0.05). These results suggest that Group 1 and 2 are high potential combination for ruminant feeds.

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CLC: > Agricultural Sciences > Livestock, animal medicine,hunting,silkworm,bee > General Animal Science > Feed
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