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Nitric oxide (NO) is a free radical nature of organisms important biological messenger molecule, has important physiological functions in the nervous, immune, blood, endocrine, respiratory, digestive and other systems, but also of cardiovascular, diabetes, oncology important factors of obesity and other diseases of the pathological process. Therefore, the detection of in vivo NO are very important in the field of life sciences, basic medicine and clinical medicine. Optical fiber sensing technology over the past several years the rise of a high sensitivity, real-time remote analysis microanalysis techniques. The technology for the information transmission capacity, immunity to electromagnetic interference, seismic, multi-component sensor integration, can be miniaturized, the unique advantages of the telemetry and in-situ monitoring represents the trend of the development of a new generation of sensors, great potential for applications in the medical, water quality monitoring, food inspection, biological reagents detection. Optical fiber sensing technology developed NO sensor, high sensitivity, fast signal transmission, environmental adaptability, easy miniaturization, is hot research scientists. This paper is in \(NO.2010-IV-050) with funding from the material design, structure and performance, bio-detection research two NO-sensitive materials: o-phenylenediamine acridine (VDABA) and 9 - (4 - ( 1,2 - di - amino) benzene-N1-phenyl) acridine (DABPA), and the sensitive material is fixed on the fiber-optic probe, to design and build a new fluorescent NO fiber optic biosensor study its sensing performance of NO . Synthesis of o-phenylenediamine the acridine (VDABA) and the main work of this paper is divided into the following areas: (1) characterize the reaction conditions (such as temperature, concentration, pH value) impact on VDABA detection of NO, measured a standard curve of the detected NO. Same time study the feasibility and sensitivity can be used to detect in vivo NO VDABA, including the detection of biological toxicity detection and cell studies show that VDABA for in vivo detection of NO. (2) Synthesis of 9 - (4 - (1,2 - diamino) benzene-N1-phenyl) acridine (DABPA), as novel NO-sensitive material, the detection of the reaction conditions (such as temperature, concentration, pH value) DABPA NO in the determination of a standard curve of the detected NO. Simultaneously study the feasibility and sensitivity can be used to detect in vivo NO in DABPA, mainly including the detection and cell detection of biological toxicity. The study showed that the DABPA for the detection of NO in vivo. (3) the design and build a new type of optical biosensor system of fluorescent type NO; VDABA into NO sensitive thin film was prepared and to determine the deposition conditions, the detection performance of the sensor at the same time conducted research, and its NO in animal serum the spiked recovery test. Study shows that the detection range of the sensor is used for NO detection of 1.0 × 10-8 ~ 1.0 × 10-5 mol / L, the detection limit is 1.0 × 10-9 mol / L, with a good linear relationship, and on animals serum accurate detection.
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