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Flower two -spotted spider mite pests of large fungal toxicity

Author: CuiYang
Tutor: YangShaoBin
School: Shenyang University
Course: Zoology
Keywords: Two -spotted spider mite Macrofungi Crude toxin Toxicity
CLC: S476
Type: Master's thesis
Year: 2012
Downloads: 41
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Abstract


Two-spotted spider mite (Tetranychus urticae Koch) is a global temperate and subtropical regions widely distributed mite is an important pest mites on flowers, one of host plants of more than 200 species and 50 families. This spider mites suck plant by sucking sap inside the leaves, flower buds endangered plants, leaves and stems, leaf plants suffer began to dry out, to China's agricultural economy incalculable losses. Through this paper, mushroom gathering, separation and purification of cultured mushroom mycelium slope, and through the mushroom product was centrifuged, broken products, crude toxin toxicity screening, selection has a significant effect Toadstool Amanita poisoning leopard and brown scales Lepiota. And fungi in two major product was centrifuged, broken product, different concentrations of crude toxin under conditions of two-spotted spider mite poison control test. Statistical results are as follows: (a) from May 2010 to October 2011 collection class has 38 kinds of mushroom species, including six kinds of mushroom, mushroom 32 kinds. Choose conducted 18 major mushroom poisoning tests. (2) on June 19, 2010 in Shenyang bird fleas collected from the market, to capture the total number is 32, of which 30 adult female, adult male 7. Male and female ratio is about 4.28:1. Will capture the two-spotted spider mite feeding laboratory, inoculated soybean leaves. A total of about 900 to 1200, reached the required number of mites. (3) through 18 major mushroom centrifugal liquid on the two-spotted spider mite adult mites and mite killing the young pilot, you can see leopard poisonous Amanita Lepiota with brown scales on the two-spotted spider mite killing effect reached 71.6%, 88.3% cytotoxicity obvious. Identification of the two mushroom toxins: leopard hallucinogenic Amanita poisonous mushroom type, does not contain toxic ingredients toxins umbrella peptide; brown scales Lepiota umbrella while containing toxic peptides and peptide two kinds of poisonous substances. Leopard rundlet crude toxin virulence test on spider mites in the first 12h, 24h, respectively when the LC50 0.120,0.075 g / ml, the concentration of 0.03,0.19 g / ml; LT50 were 35.064d, 7.783d; brown scales rundlet 24h crude toxins within the LC50 was 0.134,0.0628 g / mL, two-spotted spider mite median lethal time 0.01,0.17 g / mL concentrations LT50 were 112.136d, 8.173d. (4) on the mushroom for ultra-low temperature centrifugation, cell disruption, organic solvent extraction of crude extracts toxins on the same two-spotted spider mite concentrations 0.07g/ml different bioassay kill two-spotted spider mite. But the gap between the desired liquid multiples larger. Slide the required mushroom acaricide dipping 25-35ml, leopard umbrella, umbrella brown scales corrected mortality were 35%, 83.5%; slide capillary method required liquid 0.1ml, leopard umbrella, brown scales rundlet corrected mortality were 36.5%, 86.5%; leaf liquid immersion method required 20-25ml, leopard umbrella, umbrella brown scales corrected mortality were 43.5%, 91.5%. The slide in standard tests using a dipping method, may be used under the conditions of the test chamber slide capillary method, the field can be toxic effects leaf dipping test. (5) Strain aging effects have a significant impact on the mites. 0.07g/ml leopard poisonous Amanita same concentration Ⅳ, Ⅹ generation of strains obtained crude toxin poison against spider mites were 51.5%, 43.5%; 0.015g/ml brown scales Lepiota same concentration Ⅳ, Ⅹ generation of strains obtained crude toxin poison against spider mites were 46.5%, 26.5%, were significantly different.

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CLC: > Agricultural Sciences > Plant Protection > A variety of control methods > Biological control
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