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Blue cloth positive (Guizhou), also known as Herd seven (Shaanxi), is the rose family (Rosaceae) Roadside Green genus (Geum) roadside plant pubescent youth (Geum japonicum Thunb. Var. Chinense F. Bolle) of dried whole grass. This genus are around 70 kinds, of which our country only roadside Green (G. aleppicum), purple calyx roadside Green (G. rivale), Japan Roadside Green (G. japonicum) and pubescent roadside green ( G. japonicum Thunb. var. chinense F. Bolle) 4 种. Blue cloth is growing on a hillside meadow, gutter, bottom, flood, forest clearance and forest edge. Altitude of 200-3500 m. Folk and more used as a diuretic and astringent. As reported in the literature were obtained from a number of blue cloth middle Usu alkyl-type triterpenes, steroids and tannin compounds. Blue cloth are set out in 1977 have received version of \Therefore, from basic research and applied research perspective, there is need for a systematic blue cloth quality control standards, chemical constituents, so as to better exploitation being carried on blue cloth. The topics to July 2007 were collected from Guizhou Huaxi Gaopo blue cloth is as the research object, the various parts of its isolation and purification techniques using a variety of chemical constituents of the system separation derive 33 compounds, and the use of modern spectroscopic techniques (ESI-MS, HRESI-MS, IR, 1H-NMR, 13C-NMR, DEPT, and 2D-NMR, etc.) the structure of these compounds were identified. Identification results are as follows: * GC-1: 20? ,28-Epoxy-28-hydroxytaraxasteran-3?-Ol GC-2:? - Sitosterol GC-3: daucosterol GC-4:? - Fertility quinone GC-5: 3'-O-methyl-3 ,4-methylenedioxo ellagic acidGC-6: 3,3 '- dimethyl ellagic acid GC-7: Apocynum rather GC-8: ryegrass lactone GC-9: 7 - Poor to ryegrass lactone GC-10: p-hydroxy cinnamic acid GC-11: Ethyl gallate GC-12: megastigman-7-ene-3 ,5,6,9-tetrol GC-13: ursolic acid GC-14 : 1?, 2?, 19? - trihydroxy-3 - carbonyl -12 - en-28 - oic acid GC-15: 3-O-trans-p-coumaroyltormentic acid GC-16: acaulis acid GC- 17: 2?, 3?, 19? ,23-tetrahydroxy-12-ursen-28-oic-acid GC-18: 28 -?-D-glucoside-2?, 3?, 19?-trihydroxy-12-ursen -28-oic acid * GC-19: (7R, 8R)-4-hydroxy-9'-O-(?-L-rhamnopyranosyl) -3,3 ', 5'-trimethoxy-8-O-4'- neolignan * GC-20: (7R, 8S)-4-hydroxy-9'-O-(?-L-rhamnopyranosyl) -3,3 ', 5'-trimethoxy-8-O-4'-neolignan GC-21 : Agrimony lactone-6-O-?-D-glucopyranoside GC-22: kaempferol coumaroyl glucopyranoside GC-23: gallic acid GC-24: kaempferol 3-O-(6 ' '-OZp-coumaroyl) -?-D-glucopyranoside GC-25: astragalin GC-26: quercetin GC-27: ellagic acid GC-28: 3'-O-methyl-3 ,4-O , O-metheneellagic acid-4'-O-?-D-glucopynanoside GC-29: 3,3 '- dimethyl ellagic acid-4'-O-?-D-glucoside GC-30: nigaichigoside F1 GC -31: cupressoside A GC-32: (7R, 8S)-3-methoxy-3 ',7-epoxy-8, 4'-oxyneoligna-4, 9,9'-triol-9'-O-?-L -rhamnopyranoside * GC-33: (7S, 8S)-5-methoxycupressoside A isolated 33 compounds, including triterpenoids and steroids 10, lignans 5, gallic acid and ellagic acid derivatives 7, flavonoids 4, and others seven, including a new triterpenoids GC-1, 3 new lignan compounds GC-19, GC-20, GC-33. The four new compounds and an additional two lignan compound GC-31, GC-32 are for the first time from this genus were isolated. In order to carry out quality control of Aleppo Avens, we collected Guizhou, Yunnan and Sichuan ten samples of different origin, by comparing the \Source roadside roadside green and pubescent youth. Our medicinal properties, microscopic identification, TLC identification, moisture, ash, acid-insoluble ash, extract and gallic acid as an indicator of the determination of the items were added. In addition, all compounds of the RAW264.7 macrophage inflammatory activity of NO metabolism studies, experiments show that only a few moderate activity was detected.
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