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Effect of Exogenous Methyl Jasmonate-lanolin on Glucosinolates Accoumulation in Arabidopsis Thaliana

Author: LiuQingXia
Tutor: YanXiuFeng
School: Northeast Forestry University
Course: Cell Biology
Keywords: Arabidopsis Rosette leaves Glucosinolates Methyl jasmonate Lanolin
CLC: Q946
Type: Master's thesis
Year: 2011
Downloads: 35
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The glucosinolates (glucosinolate, mustard oil glucoside), also known as glucosinolates, a class of nitrogen, sulfur secondary metabolites, found primarily in cruciferous plants. Plants under stress, the glucosinolate-myrosinase (myrosinase) role hydrolyzed product with a variety of biochemical activity. Glucosinolates and their degradation products mediated interactions between plants and the environment, has a special role in plant defense, human nutrition and anti-cancer, thus a long time, has been one of the hot spots of attention and study. The experiment in order to dissolve the methyl jasmonate (methyl jasmonate, MeJA), lanolin (lanolin) processing rosette leaves, analysis of glucosinolates MeJA response time course, the main conclusions are as follows: (1) pure lanolin smear processing can affect the the rosette leaves glucosinolate accumulation. The aliphatic glucosinolate total pure lanolin handle 36h reaches the maximum value, and further reduced to 48h, 0h the initial level. Wherein the short chain aliphatic glucosinolate 3 - (3-methylsulphinylpropylglucosinolate methylsulfinyl propyl glucosinolate ,3-MSOP), - methylsulfinyl butyl mustard oil glycoside (4-methylsulphinylbutylglucosinolate 4MSOB) with the highest value of the content of 5 - the methylsulfinyl pentyl glucosinolate (5-methylsulphinylpentylglucosinolate ,5-MSOP) appeared at 36h after treatment, while 6 - methylsulfinyl-hexyl the glucosinolate-(6-methylsulphinylhexylglucosinolate 6-MSOH) the content of the processing time period is always lower than the content of the pre-processed in the process significantly less than the OH content of 12h and 48h, suggesting that accumulated the 3-MSOP, 4MSOB and the content of 5-MSOP by lanolin induced, while 6-MSOH accumulated significantly by lanolin suppressed. Long-chain aliphatic glucosinolate 8 - A alkylsulfinyl octyl glucosinolate (8-methylsulphinyloctylglucosinolate ,8-MSOO) content in the treated leaves always significantly higher than smear lanolin leaves content, and 8 - methylthio octyl glucosinolate (8-methylthiooctylglucosinolate ,8-MTO) within handle 36h content in the treated leaves was significantly lower than smear lanolin leaves content, that 8-MSOO accumulation induced by lanolin, while 8-MTO 36h less affected by inhibition of lanolin; indole total glucosinolate continues to rise within the processing time, 48h reached the highest value, wherein 1 - methoxy-indol-3 - methyl-mustard oil glycosides ( 1-methoxyindol-3-ylmethylglucosinolate 1MT-I3M), content processing 24h content significantly lower than before the treatment, indol-3 - methyl glucosinolate (Indol-3-yl-methylglucosinolate, I3M) the content of continuous increased and highest at 48h, 4 - methoxy-indol-3 - methyl-mustard oil glycoside (4-methoxyindol-3-yl-methylglucosinolate 4OH-I3M) and 4 - hydroxy-indole-3 - methyl glucosinolate (4-hrdroxyindol-3-ylmethylglucosinolate, 4MT-I3M) content in 36h highest, indicating the induction of various indole glucosinolates components are subject to lanolin. (2) a solution of The MeJA lanolin smear processing affect accumulation of rosette leaves glucosinolate. Lanolin dissolved MeJA the MeJA treatment group, can promote the increase in total aliphatic glucosinolate indole glucosinolate content of, both in handling 24h highest value. Dissolved the MeJA the lanolin smear treatment, short-chain aliphatic glucosinolates highest value addition to of 4-MSOB content at 36h, remaining glucosinolate content reached the highest value at 24h; long-chain aliphatic glucosinolates the-MSOO and 8-MTO content in 36h maximum; I3M the 4OH-I3M and 4MT-13M in dealing 24h achieve the highest value, while the the 1MT-13M content of the highest value in 12h. Indicate a solution of methyl jasmonate lanolin processing can promote various glucosinolates component accumulated. (3) MeJA affect the the rosette leaves most glucosinolate cumulative. Compared with the with pure lanolin handling blade, a solution of methyl jasmonate lanolin processing blade, aliphatic glucosinolate and indole glucosinolate accumulated ahead of time, reaches a maximum at 24h, and significantly higher than the content in the the pure lanolin processed leaves, indicating MeJA aliphatic glucosinolates can promote the accumulation of indole glucosinolates and total glucosinolate. Analysis showed that the case of each component by inducing aliphatic glucosinolate, several short-chain aliphatic glucosinolate 3-MSOP ,5-MSOP ,6-MSOH and two long-chain aliphatic glucosinolate 8 the the-MSOO and 8-MTO cumulative governed by MeJA induced the short chain of 4-MSOB the cumulative from MeJA induction; the cumulative four indole glucosinolates component is governed by MeJA induction, which I3M, 4OH-I3M and 4MT-I3M 24h induced the most intense the 1MT-13M MeJA-induced peak 12 hours after treatment.

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