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Studies on Morphology and Changes of Physiology-Biochemistry during Flower Bud Differentiation in Oncidium

Author: PengFang
Tutor: WeiPengXiao；TianMin
School: Guangxi University
Course: Crop Genetics and Breeding
Keywords: Oncidium Flower buds Morphological differentiation Histochemical Metabolites
CLC: S682.31
Type: Master's thesis
Year: 2011
Downloads: 26
Quote: 0
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Abstract


'Milliongolds' Oncidium (Oncidium) as test material, the use of paraffin method, the study observed the morphological characteristics of Oncidium orchid buds and bud morphological differentiation based on the leaves and flower buds of Oncidium physiological indicators measured to explore the variation of several metabolites in the flower bud differentiation process. In addition, the use of spraying different plant growth regulators Oncidium flowering. The obtained results are as follows: 1 this study Oncidium flower bud differentiation process is divided into differentiation initial period of the inflorescence primordium bud primordium calyx primordium, petal primordium co Rui column and pollen block differentiation period of six periods. 2 flower bud differentiation of three kinds of dynamic changes of metabolites in the leaves of different periods: the highest content of soluble sugar in the flower bud differentiation process sprout newborn period, after declining to reach another peak in the flower bud differentiation is completed; soluble protein content The upward trend after a first down, the protein content is the peak flowering period; total nucleic acid content of the overall downward trend. Paraffin sections periodate Schiff reaction (PAS method) and mercury bromophenol blue staining observed polysaccharides and proteins granular distribution in various organ primordia top and around. Inflorescence primordium, the content of polysaccharides in the cell is less rich in protein content and the overall distributed evenly. As the flower buds continue to divide, polysaccharides and protein content is most abundant in the calyx primordium decreased significantly in the the petal primordia period content. Enter gynostemium pollen block differentiation stage, polysaccharides and proteins in gynostemium and pollen block with the most concentrated of the Health Department. Flower bud differentiation in different periods of flower buds in the sugar and protein content changes: flower buds flower bud differentiation in different periods of total soluble sugar content began to increase into petal primordium content began to decline, after experiencing an increasing trend and rising to a peak. Soluble protein content showed a high - low - high - low trend. Oncidium flowering. Spraying different growth regulators. Results: spraying hormone (control), 200 mg · L-1 of GA3 and GA3 (200 mg · L-1) 6-BA (25mg L-1) spraying hormone treatment to make Oncidium flowering significantly advance; single 6-BA treatment flowers smaller, but the flower bud differentiation rate; flowers of GA3 6-BA treatment so larger Scape length; spraying 200mg · L-1PP333 and 250mg · L-1PP333 can Oncidium orchid postpone the extension of the treatment effect of 250mg · L-1PP333 best, can be sprayed with different concentrations of PP333 Scapes dwarf. No significant differences in the growth regulators deal with the impact of the pedicel the first pitch roughness.

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CLC: > Agricultural Sciences > Gardening > Ornamental Horticulture ( flowers and ornamental trees) > Perennial Flowers > Other Flowers > Orchids
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