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Protein as one of the building blocks of life, more and more attention, and has become an emerging discipline - proteomics, Separation and identification of hot and difficult problem faced in proteomics research, this papers will be functional nano-materials, capillary electrophoresis, and protein analysis combined, the separation of protein were studied. This thesis is divided into four chapters: The first chapter gives an overview of the functional nanomaterials, capillary electrophoresis and capillary coating basics, reviewed their recent study, described in detail the progress of applied research in nanomaterials capillary electrophoresis. Finally, the purpose and significance of this thesis. 2, the second chapter first synthesized magnetic nanomaterials with the functional groups of the epoxy group, it was characterized by infrared and scanning electron microscopy. Infrared spectra show that the functional magnetic material with an epoxy group has been successfully synthesized, scanning electron microscope showed that the average particle diameter of 160 nm. Then through cooperation with chemically synthesized with epoxy-functional nano material coating to the capillary wall, sodium diacetate and metal ions then imino bond is bonded to the capillary wall, to prepare a coated capillary of the fixed metal ions nanomaterials with streaming potential and inverted fluorescence microscopy to characterize. Different pH values ??(pH 3 to 9) Capillary: bare column APTMS modified capillary Fe3O4 _AT_ SiO2 _AT_ GLYMO nano-particle modified capillary and Fe (III) - nanoparticles chelate coated capillary flow potential The figure below illustrates the Fe (Ⅲ) - nanoparticles chelate successfully successfully coated on the capillary wall, and its repeated measurements illustrate capillary coating stability. Fe (Ⅲ) - inverted fluorescence microscope diagram many bright spots, both Morin-Fe3 complex nanoparticles chelate coated capillary. 3, Chapter on the basis of the previous chapter, chelate metal ions nanomaterials coated capillary as the electrophoresis channel to establish a new method of capillary electrophoresis separation of phosphorylated proteins and non-phosphorylated proteins. The results show that under optimum conditions, bovine serum albumin, beta-casein, hemoglobin, ovalbumin protein can be obtained a good separation of the four protein peaks order non-phosphorylated proteins, first-out peak phosphorylated proteins After the peak, the number of theoretical plates of the respective proteins were 29 000 / m (hemoglobin) 42 000 / m (bovine serum albumin), 32000 / m (ovalbumin) and 233 000 / m (the p-casein ). Reproducibility of the separation of the coated capillary were investigated eight consecutive experiments, under the same conditions, only a slight change in the migration time of the various protein, specifically: hemoglobin (0.72% RSD), bovine serum albumin ( 1.48% RSD), ovalbumin (2.57% RSD) and p-casein (0.89% RSD). To further verify the validity of the separation of the coated capillary, applied to complex phosphorylated proteins phosphorylated protein mixture - milk protein separation. The results show that the five proteins can be obtained a good separation of the order non-phosphorylated proteins Five protein peaks (α-lactalbumin and p-lactoglobulin), first-out peak phosphorylated proteins (α-casein, after the peak in the β-casein and κ-casein). 4, Chapter IV Conclusion and Outlook.
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