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Borate nanomaterials enrichment glycopeptides or glycoproteins

Author: ZhangHuaiYuan
Tutor: YangPiYuan
School: Fudan University
Course: Biomedical
Keywords: Biological Mass Spectrometry Proteomics Separation and enrichment Glycopeptide Glycoprotein Nanomaterials
CLC: TB383.1
Type: Master's thesis
Year: 2009
Downloads: 156
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Abstract


Recently, with the rapid development of proteomics ( proteomics ) , sugar proteomics have emerged , and gradually the field of life science research and a new frontier and hot . In this thesis, biology, chemistry, materials science and other interdisciplinary blend into the background, to explore the synthetic borate nanomaterials research enrichment of glycopeptides or glycoproteins . Although there have been many methods and materials are used in the enrichment of glycopeptides or glycoproteins , glycopeptides or glycoproteins enrichment analysis within the technical field of a long and a huge challenge . Therefore, the development of other new enrichment techniques and methods is important and urgent . This paper consists of four parts . The first chapter introduces the latest progress of the sugar proteomics , focusing on the forefront of the development of biological mass spectrometry and its applications in proteomics and glycopeptides or glycoproteins separation and enrichment of the new strategy , new technologies and the identification of common glycoprotein / method for determining glycosylation sites . Chapter II : Synthetic a functional 4 - Mercaptophenylboronic acid gold nanoparticle material . Because it glycopeptide or glycoprotein cis 1,2 - dihydroxy chemically react to form a covalent bond , to be applied to the separation and enrichment of glycopeptides or glycoproteins . The material on glycopeptide or glycoprotein enrichment achieved very good results. Chapter III : Synthetic Another new functional mesoporous nano - magnetic material borate . Because the material has a very large surface area and superb magnetic , so it's enrichment efficiency is very high . Also achieved very good results on the standard glycopeptide or glycoprotein enrichment . Chapter : Golden Globe boric acid magnetic nanomaterials enrichment of glycopeptides or glycoproteins . Standard glycopeptides and glycoproteins also achieved very good results , it has the potential to become a useful and powerful tool in the research field of the glycoprotein of the group .

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