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The Study on Forsythiaside a Inhibit IBV and Regulat the Expression of IFN-α Signaling Pathway-related Factors

Author: LiHuaWei
Tutor: WuGuoJuan
School: Beijing University of Agriculture
Course: Basic Veterinary Science
Keywords: Forsythoside A IBV IFN-α CEK cells Positive regulation
CLC: S853.7
Type: Master's thesis
Year: 2011
Downloads: 31
Quote: 0
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Abstract


Objective:In this study, to investigate the effect of Forsythoside A inhibiting avian infectious bronchitis virus (IBV) in vitro,the chick embryo kidney (CEK) cell culture model was established, and The expression of IFN-αand related factors of JAK-STAT signaling pathway were investigated when forsythoside A put on CEK cells.Methods:The standard strain of IBV-M41 was used to infect chicken embryo kidney cells, to detect the effect of forsythoside A inhibit IBV the content of IBV N gene was detected by real time quantitative PCR and cytopathic (CPE) observation; And, to judgement the effect of forsythoside A inducing the expression of IFN-a and factors of JAK-STAT signaling pathway, the expression of IFN-a and related factors of JAK-STAT signaling pathway were observed by real-time quantitative PCR, furtherly, the protein expression levels IFN-a was determined by western-blot in this study.Results:The results show that when IBV treated with forsythoside A in the concentration of 0.64mM directly,it can inhibit IBV completely after 36h, and 0.64mM forsythoside A pretreatment CEK cells can inhibit IBV partially; and compared with the normal group, produced the expression of IFN-a, and related factors of JAK-STAT signaling pathway were detected after high-dose forsythoside A was added to CEK cells at 12 hours.Conclusion:When forsythoside A in a certain concentration range, the replication of IBV can be inhibited, and it can also induce IFN-a and regulate the JAK-STAT signaling pathway positively.

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CLC: > Agricultural Sciences > Livestock, animal medicine,hunting,silkworm,bee > Animal Medicine ( Veterinary Medicine) > Chinese veterinary science > Veterinary Pharmacology
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