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Somatic Embryogenesis and Cell Suspension Culture Systems of Juglans Mandshurica

Author: JiangSiJia
Tutor: WangFei；ZhangJianZuo
School: Northeast Forestry University
Course: Ornamental Plants and Horticulture
Keywords: Juglans mandshurica Somatic embryos Suspension culture
CLC: S792.132
Type: Master's thesis
Year: 2011
Downloads: 49
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Abstract


Of Manchurian walnut (Juglans mandshurica Maxim), are deciduous tree Juglandaceae, produced in the north of the Yellow River basin and the northeast region. Walnut catalpa wood fine is known as one of the the northeast three hardwood (Ash, walnut, yellow pineapple). Walnut catalpa tree beautiful viewing and has good resistance and patience, and can absorb industrial emissions to improve the living environment of human beings, so in recent years is widely used in landscaping, green street line planting and small environment piece sik. In addition Juglans mandshurica also has high medicinal and industrial production value. Juglans mandshurica resources in recent years, over-exploitation and seed bank low reproductive rate and other causes lack of Juglans mandshurica forest resources. Embryo Culture for the conservation and utilization of germplasm resources provides new ways. This thesis carried out on the of Juglans mandshurica somatic embryos occurred system and the establishment of embryogenic cell suspension cultures in-depth research, Juglans mandshurica fast breeding, seed cultivation, development of somatic embryos bioreactor production technology as well as forest species genetic engineering research laid the foundation for the main findings are as follows: 1. embryogenic callus induction of Manchurian walnut optimum conditions: ① zygotic embryo explants induced embryogenic callus, explants drawn during the May-June (immature embryo) the zygotic embryos callus induction rate up to 91.3%. ② walnut catalpa embryogenic callus induction medium MS 1.0mg L-12 ,4-D 0.5mg · L-16-BA additional sucrose 30g / L agar 6g / L, the optimum. (3) somatic embryogenesis induction, development and differentiation of the suitable medium for the MS basic medium supplemented with sucrose 30 g · L-1, casein hydrolyzate 700mg · L-1, agar 6g / L and does not add any growth regulators. 2. Suspension culture best breeding medium 1/2MS 0.05mg / L 2,4-D liquid medium; the best somatic embryos development the medium for 1/4MS not add any liquid medium growth regulators; most Jia inoculation density of 7g / L, the This concentration only conducive for the proliferation, there are conducive to the growth and development. 3 in Juglans mandshurica suspension cell culture medium, the trend of the pH value was \dropped to 4.21, and the slow growth of cell dry weight; 12d culture liquid average pH value began to rise to 5.3, cell dry weight fast growth; 15d after the end of the cell growth, the average pH of the culture medium is maintained at about 5.44, dry cell re-stabilized. 4 in the process of cell suspension cultures, ① 30g / L sucrose concentration conducive to cell proliferation and growth, the sucrose concentration is too high or too low to affect cell growth. (2) suspension culture cells growth cycle in the 15d. ③ different sucrose concentration suspension culture, of Juglans mandshurica cells the rate of hydrolysis of sucrose with sucrose concentration increase time growth. The ④ cells reducing sugars with sucrose content increases the difference in culture medium gradually decreases

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CLC: > Agricultural Sciences > Forestry > Forest tree species > Broad-leaved trees > Walnut > Juglans mandshurica
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