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Of Huang Bo (Phellodendron amurense Rupr.) Rutaceae broad-leaved trees, mainly distributed in the northern region in our country. Due to the demand for traditional Chinese medicine and modern pharmaceutical, wild Huang Bo resources have been severely damaged, have been listed as two national protected plants. Previous studies have reported that, of Huang Bo suffered a serious pest invasion, affected the normal growth of the trees. Therefore, the study of Huang Bo defense mechanism is particularly important, the current study reported more medicinal ingredients of Huang Bo, but the chemical ecology and plant physiology perspective of gender, age, health status, spatial location and growth stages of Huang Bo resistance The lack of the relationship between the substances reported. 37,56 years old of Huang Bo from the the defense protein distribution in the the Phellodendron amurense plants within the phenolic acids gender differences, age differences, health disparities and seasonal variation of the systematic research to explore Phellodendron amurense defense mechanism of resistance, provide some basic data for the protection and utilization of the resources of Huang Bo. The results are as follows: a different growth period of Phellodendron amurense health and victimization plant defense protein activity difference analysis of the different growth stages of Huang Bo healthy and victimized male and female strains of fruit, leaves and twigs defense protein activity of the timing change and health and victimization plants of differences between defense protein activity using ultraviolet spectrophotometry defense proteins - enzymes of protective, defensive and trypsin inhibitor activity. The results show that the defense protein activity showed July gt; August gt; June, and the differences between the different months are significantly between healthy and damaged plant defense protein activity did not differ significantly (addition to POD7 month leaves a significant difference outside). 2 different ages of Huang Bo male and female plants defense protein activity space differences in measured three months, two different old health and the protection of victims of female plants of enzymes and PPO activity showed the highest of the trees, the leaves, followed by the fruit of the lowest , the male plant branches protection enzymes and PPO activity were significantly higher than the activity of the leaves; PAL activity of the female plants are showing the fruits of the highest, followed by the branches, leaves the lowest male plants leaves PAL activity were significantly higher than the branches of activity ; female plants of TI activity showed the highest leaves, branches, followed by the fruit of the lowest, male plants branches TI activity were significantly higher activity than leaves. The female plants TI activity was significantly lower than the activity of the male plants while the rest of the defense protein activity were significantly higher than the activity of the male plants. The differences were between the two old defense protein activity significantly, and the 37-year-old Huang Bo significantly higher than the 56-year-old of Huang Bo. Different Age 3 different age leaves the of Phellodendron amurense health and victimization of male and female plants within the phenolic total variance analysis of the difference of the total phenolic acids within the of Phellodendron amurense health and victimization of male and female plants leaves, phenolic acid content using high performance liquid the ยจ chromatography method analysis A total of protocatechuic acid, chlorogenic acid, caffeic acid, coumaric acid, salicylic acid, benzoic acid, phenylacetic acid, ferulic acid and cinnamic acid phenolic acids, phenolic damaged plant species more than healthy plants. The results show that the phenolic total showed July gt; August gt; 6 months, and the differences between the different months are significant difference between healthy and damaged plant phenolic total significantly. Measured three months, the female plants the total amount of phenolic acids were significantly higher than male plants, the difference between the two small old phenolic total were also significant, and was significantly higher than the 56-year-old to the 37-year-old Phellodendron amurense Phellodendron amurense. The results of this study show that, gender, age, spatial location and growth period of Huang Bo plant defense protein's accumulation affect the differences are significant with (P lt; 0.05), the health status of their influence differences are not significant with; gender, age, health status and growth period of Huang Jie amurense leaves phenolic total accumulated impact of the differences were significant (P <0.05). This paper is the first to compare systematically studied and expounded gender, age, health status, spatial location and growth stages of Phellodendron amurense defense capability for different gender, different ages of Huang Bo plantations scientific management and breeding of fine species new theoretical basis for the implementation of Huang Bo Luoli Psylla ecological control and reduce chemical control on the environment caused by hazards, has an extremely important significance. In the present study, the activity of the resistance-associated substances in different spatial position (location) of Huang Bo between fluctuations obvious regularity, and species characteristics and other factors, which may need to be increased in future studies of Huang Bo biometrics further substances variation of the resistance of Huang Bo Huang Bo Luoli psyllid population trend analysis in order to provide a more reliable theoretical basis.
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