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Premature aging of cotton leaves directly lead to premature aging of the cotton plant cotton premature aging must be accompanied reduce the strength of cotton fibers , fiber uniformity decline , shorter fiber length , resulting in reduced quality of fiber into yarn . Therefore, the study of cotton leaf senescence mechanism , has important significance to improve cotton production to improve the quality of cotton fiber . Cotton Leaf Senescence and functional genomics research has made ??some progress in proteomics research , however , is still in its infancy . In this study, two-dimensional electrophoresis proteomics combined with mass spectrometric identification method , a comparative analysis of two cotton material . Premature aging materials CCRI 10 and CCRI 16 blades not premature material protein sample was extracted total protein were separated by two-dimensional electrophoresis technology , Coomassie blue staining , scanned dimensional electrophoresis profiles using the software ImageMaster 2D Platinum 7.0 Analysis MALDI-TOF-TOF mass spectrometry identification of differentially expressed proteins selected protein spots . During the experiment, using the improved Coomassie Brilliant Blue CBR250 staining technique on polyacrylamide gel was stained , the obtained spectra clearly lower background and detects more protein points , effect is ideal . Successfully identified 92 protein spots , including 40 in the 10th , premature aging materials CCRI down-regulated expression , 52 upregulated . Get protein functional classification : accounting for 47.44% of all identified proteins involved in carbohydrate transport and metabolism ; involved in protein translation modification , protein folding and molecular chaperone protein accounted for 19.23% of all identified proteins involved in energy metabolism and transporter protein accounted for 12.82% and inorganic ion transport and metabolism accounted for 10.26% , 2.56% amino acid transport and metabolism , as well as some unknown protein involved in protein and other metabolic processes function . Six differentially expressed proteins from the gene level validation using RT-PCR method , the result is consistent with the results of part of the two-dimensional electrophoresis . The experimental results show that proteomics is an effective means to study differences in protein during leaf senescence , and to lay the foundation for research as well as premature aging problems of cotton leaf senescence mechanism .
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