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Pinellia Rooting Experiment of Tissue ternata Berit Araceae perennial herbs of medicinal plants, tubers medicine, traditional Chinese herbal medicines. Pinellia species degradation caused due to a virus often leads to lack of seed source, the yield and quality of a serious decline, has become one of the main obstacles in Pinellia production, removal the Pinellia seedlings and Microtuber of viral culture through the apical meristem and other means of biotechnology is the way to resolve artificial seed production and increase production quality. In this paper, by different plant growth substances on callus induction studies synchronization through of Pinellia suspended cells for the production of artificial seed the small tubers material to provide a large number of synchronization, and of Pinellia artificial seed technology research provide theoretical and technical foundation for of Pinellia artificial seed production and application. The main findings are as follows: (1) Pinellia petiole explants, 2,4-D orthogonal design of NAA, picloram and KT 4 plant growth substances and proportion of loose callus formation impact. The results show that :2,4-D and picloram Pinellia petiole callus induction effect of the most significant of KT and NAA induced effect, followed by induction Pinellia petiole formation of loose callus suitable medium for the MS 2,4 - D 0.5 mg · L -1 NAA 1.0 mg · L -1 picloram given 1.0 mg · L -1 KT 1.5mg · L -1 . (2) to the clover Pultaceous callus for material transferred to the MS 6-BA 0.5 mg · L -1 2,4-D 1.0 mg · L -1 CH300 mg · L the -1 in the liquid medium and cultured for 3-4 times, and then measured the growth cycle of the cell suspension 15-21 d Pinellia suspended cells in the exponential growth phase, At this point the best cultured cells subculture time. (3) different plant growth regulator substances affect the ratio of cultured of Pinellia suspended cells, studies have shown that the best suspension liquid medium MS 2,4-D 0.5 mg · L -1 6 -BA 0.5 mg · L -1 . (4) The orthogonal design method to study the low-temperature processing of the the Pinellia suspension cultured cells synchronized, and the resulting data range analysis and variance analysis. The results showed that the cold treatment, the mitotic index has improved to varying degrees, which temperature 8 ℃ mitotic index is greater than the other two groups of temperature treatment of cell division index, while the in 8 ℃ under low temperature conditions, processing 24 h recovery at 36 h, and the effect of synchronization best mitotic index was up 11.3%. (5) after synchronization of Pinellia suspended cells materials, design the orthogonal experimental L 9 (3 4 ), to study different concentrations of 6-BA, KT NAA and 2,4-D cell clusters differentiated small tubers, filter out the most suitable for the differentiation of the the small tubers liquid medium MS 6-BA0.5 mg · L -1 NAA0.5 mg · L -1 2,4-D 0.25 mg · L -1 sucrose 30 g · L -1 . (6) suspension culture differentiation of Pinellia small tubers embedded material, different concentrations of chitosan, polyethylene glycol, of Carbendazim and sodium benzoate germination rate of of Pinellia artificial seed production and seedling the rate impact, the results show that, the Pinellia trades of leather for a 3% sodium alginate 2% CaCl 2 0.05% CoCl 2 0.05% CuCl 2 0.1 mg · L -1 GA 3 4% chitosan 2% polyethylene glycol 0.4% sodium benzoate best germination rate of 85%; screening Pinellia person trades of leather 2 0.05% CuCl 2 0.1 mg · L 2 0.05% CoCl > -1 GA 3 4% chitosan 4% polyethylene glycol 0.2% carbendazim, the seedling rate up to 95%.
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