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The Research of Flavonoids Contents and Allelopathic Effects of Oxytropis Glabra DC. in Ordos Grassland

Author: LuoYang
Tutor: LiaoRongSu
School: Beijing Forestry University
Course: Ecology
Keywords: Oxytropis glabra Different growth stages Total flavonoid content Extraction Process Allelopathy
CLC: S452
Type: Master's thesis
Year: 2011
Downloads: 56
Quote: 1
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Abstract


Flavonoids are a class of secondary metabolites produced in the process of plant growth, as one of the main active ingredient of the medicinal plants and flavonoids, with a variety of pharmacological effects. Oxytropis glabra arid steppe toxic medicinal plant, widely distributed and abundant resources. Oxytropis glabra flavonoids in order to effectively use ethanol as a solvent extraction of total flavonoids of Oxytropis glabra, total flavonoids content was determined by spectrophotometry. Through single factor test the effects of ethanol concentration, extraction time, solid-liquid ratio, extraction temperature of four factors on the extraction rate, based on the orthogonal experiment to determine the optimum extraction conditions for: ethanol concentration of 70%, the extraction time of 2.5 hours, the water bath extraction temperature 80 ° C, the solid-liquid ratio 1:60. Oxytropis glabra samples of different growing season from May to September flavonoids extracted, and its content was determined using optimum extraction conditions. The results show that: Oxytropis glabra total flavonoids in September, the highest content reached 13.0 mg / g, 8 content is lowest in January to 6.2mg / g, the dynamic changes in trends of different growing season Oxytropis glabra flavonoids September gt; January gt; to in June gt; gt in July; in August. Determination of total flavonoids of Oxytropis glabra underground parts (roots) mean to 4.3mg / g. Flavonoids are important allelochemicals one in order to understand the effects of Oxytropis glabra Allelopathy Allelopathy and flavonoids in other plants, the use of biological test method to study the aerial parts of Oxytropis glabra Allelopathy and aqueous extracts of the underground part of the pasture, grain crops and vegetables, 11 kinds of plant seeds germination and seed vigor. The results show that most of the seeds in the of Oxytropis glabra different concentrations of aqueous extracts of cultured, roughly in line with the increased concentrations of aqueous extracts germination suffered inhibition law; radish seeds flowers Oxytropis water the immersion response to the outstanding performance of low concentrations promote high concentration inhibition; addition, cucumber, cabbage, canola, sorghum, wheat and other seeds in low concentrations of aqueous extracts of germination and growth is also a slight role in promoting . Overall, the aerial parts of Oxytropis glabra Allelopathy Allelopathy stronger than the underground part of the aerial parts contain more flavonoids.

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CLC: > Agricultural Sciences > Plant Protection > Harmful plants and their removal > Poisonous weeds
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