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Our potato acreage and production rank first in the world , but the potato disease has been threatening the development of China 's potato industry . Potato eubacteria disease : potato late blight (Phytophthora infestans (Mont.) deBary), potato dry rot ( of Fusarium spp . ) , Potato mole disease (Rhizoctonia solani Kuhn), potato soft rot ( bacterial genera such as Erwinia spp . ), potato bacterial wilt (Ralstonia solanacearum) . Improve the quality of seed potatoes , detecting and removing substandard seed potatoes contain potentially pathogenic hazards , current prevention and control methods . This requires the development of a fast and efficient disease detection methods for strict monitoring of seed potato production to ensure the quality of the seed . The latest developments in Real Time PCR has the advantages of simple operation , quick and easy , high sensitivity , good repeatability , low pollution , is widely used in pathogen detection . The above experiment was based on several important potato eubacteria pathogens conserved sequences designed Taqman probes and primers . Potato late blight (Pi), of mole disease (Rhs) and soft rot (Ec) probe JOE fluorescently labeled stem rot disease ( Fz ) , bacterial wilt (Rs) and potato reference gene ( cytochrome oxidase Cox ) probe with FAM-labeled . Potato internal reference COX introduced in the detection process can effectively avoid false negative results due to the failure of DNA extraction or PCR inhibition resulting . With fluorescent dye SYRB GREEN I and the labeled Taqman probe real-time quantitative PCR experiments were different double probe Real time PCR and probe fluorescence marker . The dual combination of quantitative PCR : Rhs Cox, Fz and Ec , Pi and Rs ; Pi Fz can not combine . The the comparative fluorescence quantitative detection method with ordinary PCR detection sensitivity than conventional PCR , quantitative PCR sensitivity 10 to 100 times higher . This approach combines the advantages of multiplex PCR and quantitative real-time PCR , high sensitivity , specificity , reduce costs , save time , and provide a reliable guarantee for virus-free seed potato production quality .
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