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Part I: plasma DNA of patients with acute pancreatitis quantitative analysis and clinical significance of the purpose of quantitative detection in peripheral blood of patients with acute pancreatitis the plasma DNA content and explore its clinical value. Collected 40 patients with mild acute pancreatitis (MAP) in peripheral venous blood of 20 patients with severe acute pancreatitis (SAP), and 50 cases of healthy persons, \PCR was used to detect the of plasma DNA content, and comparative analysis of the clinical relevance of plasma DNA levels with acute pancreatitis, and as a receiver operating characteristic (ROC) curve. Results (1) SAP group, the median plasma DNA content of the MAP group and the healthy control group were 24.84ng/ml, 7.60ng/ml 5.23ng/ml, the difference between the three groups was statistically significant (P = 0.000 ); (2) 60 patients with acute pancreatitis plasma DNA content was significantly higher than the healthy control group (P = 0.006); SAP group was significantly higher than the MAP group and the healthy control group (P = 0.006, P = 0.000), while the MAP group with no significant difference between the healthy control group (P = 0.322); (3) SAP group and MAP group plasma DNA content by the receiver operating characteristic (ROC) area under the curve of 0.881 (95% confidence interval for 0.773 to 0.989), to plasma DNA ≥ 11.20ng/ml as the critical value the the plasma DNA diagnosis of severe acute pancreatitis sensitivity 95%, specificity of 72.5%; (4) plasma DNA levels in patients with acute pancreatitis, APACHE - score, Ranson score, serum Ca2 concentration, serum CRP levels is closely related (P = 0.001, P = 0.013, P = 0.000, P = 0.001), and with the number of days in hospital and peripheral blood leukocyte number was no significant correlation (P = 0.215 , P = 0.161). Conclusions plasma DNA levels with acute pancreatitis is closely related to the severity of the patient's condition, may be an important indicator of acute pancreatitis patient's condition monitoring and early diagnosis of severe acute pancreatitis. Part II: Gene expression profiles of peripheral blood mononuclear cells of patients with acute pancreatitis purpose of analysis of gene expression changes in peripheral blood mononuclear cells in patients with acute pancreatitis, to explore the molecular mechanisms of severe acute pancreatitis. Severe acute pancreatitis (SAP), mild acute pancreatitis (MAP) and healthy persons as research subjects were collected peripheral venous blood, peripheral blood mononuclear cells isolated by Ficoll density gradient centrifugation, extraction of total RNA, using cDNA microarray Affymetrix U133A 2.0 and data processing software SAP patients were screened than MAP patients and healthy persons differentially expressed genes in the intersection of the analysis, the two sets of data, and differences in gene common change as SAP-related gene expression patterns, application of bioinformatics methods to analyze and use the Real-Time PCR method to verify some of the screening results. SAP and healthy comparison results of differentially expressed genes (2-fold) of 2574, 1193 upregulated genes down-regulated genes 1381; compare SAP with MAP, 755 differentially expressed genes, which raised 480 genes, 275 down-regulated genes. Intersection analysis, screening of 413 SAP related significantly differentially expressed genes, gene expression patterns as SAP, including 305 upregulated genes and 108 downregulated genes, the function of these related genes involved in biological regulation, stress signal processing , immune response, cell death, cell adhesion, and other aspects. Conclusion Microarray initial screening of candidate genes differentially expressed genes between SAP and healthy, to investigate the pathogenesis of SAP. While filtering out the difference between the SAP and MAP expressed genes after intersection analysis of SAP-related gene expression patterns, consider this part of the gene may occur, with SAP development are closely related.
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