Dissertation > Excellent graduate degree dissertation topics show

The Changes of Intestinal Barrier Functional in Rats with SAP and the Effection of QingYi Ⅱ

Author: CaiZhiFang
Tutor: DuiDanHua
School: Zunyi Medical College,
Course: Surgery
Keywords: Severe acute pancreatitis Diamine oxidase D-lactate Intestinal--barrier functional Traditional Chinese medicine QingYiⅡ
CLC: R657.51
Type: Master's thesis
Year: 2011
Downloads: 1
Quote: 0
Read: Download Dissertation

Abstract


Objective:To observed the injury of intestinal barrier function in rats with SAP.and explored the effection of the traditional Chinese Medicine QingYi II on rats with severe acute pancreatitis complicated with intestinal barrier functional distur-bance (IBFD). which provided experimental basis for QingYiⅡto further clinical development and applications.Methods:104 healthy SD rats were randomly divided into five groups:sham operation group (A group, n=8);SAP model group (Bgroup, n=24); QingYi II-treated group (Cgroup, n=24); Glutamine-treated group (Dgroup, n=24); QingYiⅡand Glutamine-treated group(Egroup, n=24). Animals in group A were only flipped the intestinal canal; SAP model was established by retrograde injection of 3% sodium taurocholate into bilio-pancreatic duct of rats in group B、C、D、E.Rats in Group A、B were fed with saline; the group C were fed with QingYi II and the group D were fed with Glutamine.the group E were fed with QingYi II and Glutamine. after SAP model had been established,rats were sacrificed respectively at 6h、12h and 24h.blood, pancreatic and intestinal tissue samples were clollected. the Concentrations of serum-AMY、TNF-a、DAO、D-lactate were measured.Pathologic change of intestinal and pancreatic were observed by microscope.The ileum were observed by electron microscope.Results:Compared with the group A, the ascites、serum-AMY、TNF-a、DAO、D-lactate in group B、C、D、E were increased significantly at 6h、12h、24h (P<0.01),and those were diffrences significantly at 6h、12h、24h in respectively group B、C、D、E. Compared with the group B, the ascites、serum-AMY、TNF-a、DAO、D-lactate in group C、D、E decreased at 6h、12h、24h (P<0.05), and there were diffrences at 6h、12h、24h but the ascites. Compared with the group E, the ascites、serum-AMY、TNF-a、DAO、D-lactate in Group C and D were increase at 6h、12h、24h (P<0.05).Compared with the group A, The Pathological scores of pancreas in group B、C、D、E increased at 6h、12h、24h (P<0.01);Compared with the group B, The Pathological scores of pancreas in group C、D、E decreased at 6h、12h、24h (P<0.05), The Pathological scores of pancreas were not diffrenct between group C and group D; but that in group E was less (P<0.05).The Pathological scores of the ileum in Group B、C、D、E were higher than that in Group A at 6h(P<0.05);Compared with the group B, The Pathological scores of pancreas in group C、D、E were significantly decreased at 12h、24h (P<0.05), and notdiffrence between group C and groupD(P>0.05); that in group E was less than group C at 6h、12h、24h (P<0.05).The intestinal microvilli is clear, the number of multiple,vertical,raised and arranged in neat in group A;the intestinal is ischemia,necrosis,loss of microvilli in group A,the intestinal microvilli in group C is less than that in group A,and more than group B. its surface microvilli atrophy got short and arranged less neatly. Transmission electron microscopy showed the A group of intestinal epithelial endoplasmic reticulum, mitochondria slightly swollen microvilli normal structure in group A; microvilli damage, swelling, swelling of intestinal epithelial cells significantly, showing early apoptotic cells in group B.Conclusion:There is the injury of intestinal barrier function in rats with SAP. The traditional Chinese medicine QingYiⅡplayed an important role in prtecting in rats SAP complicated with IBFD.Combinating QingYiⅡand Glutamine is better.

Related Dissertations

  1. Research the Relevant of Detection the Index of Rabbit Model of Intra-abdominal Hypertension and Intestinal Barrier Function.,R656
  2. Bone marrow mesenchymal stem cells in rats with severe acute pancreatitis CD4 ~ CD25 ~ regulatory T cells influence and Integrative Medicine,R576
  3. Rhubarb , magnesium sulfate treatment of severe acute pancreatitis comparative study of the clinical efficacy,R576
  4. The Study on Monocarboxylate Transporter (MCT1) Expression Level of Rat Cardiac Myocardial Cells and Fibroblasts during Ischemia and Reperfusion,R614
  5. Protective Effect of Edaravone in the Intestinal Mucosal Barrier Injury of Severe Acute Pancreatitis in Rats,R965
  6. The Treatment of Low-dose Glucocorticoid to Severe Acute Pancreatitis: An Clinical Observation,R576
  7. Studies on the Protective Effect on Partial Ogans of Rats with Severe Acute Pancreatitis and Its Expression of Aquaporin-4 by Qingyi Grains,R285.5
  8. Ultrasound-guided catheter lavage in the treatment of severe acute pancreatitis Application Value of,R576
  9. Discussion Qing Yi Decoction Treatment of Severe Acute Pancreatitis Mechanism and Clinical Analysis,R576
  10. Singnificance of High-Mobility Group Box Chromosomal Protein-1 in Severe Acute Pancreatitis and the Protective Effect by Somatostatin Combined with Growth Hormone,R576
  11. Death in patients with severe acute pancreatitis risk factors,R576
  12. Effect of Curcumin on Acute Lung Injury in Severe Acute Pancreatitis in Rats,R285
  13. Effect of XueBiJing Injection on Serum Levels of High Mobility Group Box Protein 1 and Heat Shock Protein 70 in Severe Acute Pancreatitis Rats,R285.5
  14. Study on Cellular and Molecular Mechanisms of Brain and Kidney Injury Induced by Lead Acetate,R114
  15. Study on Solid Acid Catalyst and Process of Synthesis N-Butyl Lactate,TQ225.241
  16. Therapeutic Trial for Piglet’s Yellow-white Dysentery with Lactic Acid Ciprofloxacin,S858.28
  17. Influence of Thymopentin Resuscitating Traumatic Hemorrhagic Shock on T-lymphocyte Subpopulation and Intestinal Mucosal Barrier in Rats,R605.971
  18. A Meta Analysis of Early Enteral Nutrition Versus Total Parenteral Nutrition in Patients with Severe Acute Pancreatitis,R657.51
  19. The Analysis on Diagnosis and Treatment of Pancreatic Pseudocysts Resulted from Severe Acute Pancreatitis,R657.51
  20. The Change of Nitric Oxide Synthase Neurons in Enteric Nervous System in Severe Acute Pancreatitis Rat,R576

CLC: > Medicine, health > Surgery > Of surgery > Abdominal surgery > Pancreas > Pancreatitis
© 2012 www.DissertationTopic.Net  Mobile