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Objective To observe Xuebijing Injection (XBJ) the impact of severe acute pancreatitis (SAP) the serum HMGBl and HSP70 , to explore the role XBJ the treatment of severe acute pancreatitis . Methods 60 male SD rats were randomly divided into four groups , sham-operated group (A ) , SAP group (B ) , SAP XBJ (4 mL / kg) low-dose treatment group (C ) the SAP high dose XBJ (8 mL / kg) treatment group (D ) . Using 5% sodium taurocholate retrograde cholangiopancreatography injection rat model of SAP , C group after intraperitoneal injection immediately XBJ ( 4 mL / kg ) , D group after intraperitoneal injection immediately XBJ ( 8 mL / kg ) , 12h repeated injections . Each group was randomly divided into 6,12 h.24h3 subgroup , after rats were sacrificed at each time point , using the enzyme-linked immunosorbent assay (ELISA) in the serum levels of HMGB1 and HSP70 . Results 1.A group at each time point HMGB1 concentration no significant change ; B , C, D serum HMGB1 concentration after 6h no significant changes , no significant difference compared with group A ; B , C , D group postoperative 12h have significantly increased , 24h increased further, the group A with the same time point , there was a significant difference ( P lt ; 0.05 ) . C, D group 12h , 24h concentration was significantly lower than the same time point in group B ( P lt; 0.05 ) ; Group D 12h, 24h concentration of group C was significantly lower than the same time point ( P lt; 0.05 ) . 2.A group at each time point serum HSP70 concentrations were lower ; B , C, D serum HSP70 concentration after 6h were significantly increased with time , the concentration gradually increased , and at the same time point A group there was a significant difference ( P lt; 0.05 ) . C, D serum HSP70 concentration significantly lower than the same time point in group B ( P lt; 0.05 ) ; and D serum HSP70 concentration was significantly lower than the same time point in group C ( P lt; 0.05 ) . Conclusion HMGB1 may be as late inflammatory cytokines involved in SAP systemic inflammatory response ; extracellular HSP70 may as the proinflammatory cytokine involved in systemic inflammatory response in the SAP ; Xuebijing SAP can be significantly lowered serum levels of HMGB1 and HSP70 severe acute pancreas inflammation has protective effects in rats .
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