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Background Acute pancreatitis is an inflammatory disease, severe acute pancreatitis often and early involvement of the lungs. Excessive release of inflammatory cytokines and uncontrolled systemic inflammatory response, systemic inflammatory response pancreatitis has become the core of the problem. HMGB1 as a late inflammatory mediators involved in the systemic inflammatory response of severe acute pancreatitis. Xuebijing injection is our country has passed the stage Ⅱ, Ⅲ clinical trials of new Chinese medicine treatment of sepsis, the most prominent is a potent anti-endotoxin by potent endotoxin-induced monocyte / macrophages produce endogenous uncontrolled release of inflammatory mediators role. HMGB1 as a late inflammatory core media may also become a target for Xuebijing role, while Xuebijing by reducing serum HMGB1 levels to play lung protection. Objective To observe Xuebijing injection (XBJ) on severe acute pancreatitis (Severe acute pancreatitisSAP) serum high mobility group protein -1 (high mobility group box protein 1, HMGB1) explore the effect of the treatment of severe acute pancreatitis XBJ lung injury. Methods 60 male SD rats were randomly divided into four groups, sham operation group (A group), SAP group (B), SAP low dose XBJ (4mL/kg) treatment group (C group), SAP high dose XBJ ( 8mL/kg) treatment group (D group). With 5% sodium taurocholate retrograde cholangiopancreatography injection model of SAP rat was made, C group immediately after intraperitoneal injection XBJ (4mL/kg), D group immediately after intraperitoneal injection XBJ (8mL/kg), 12h repeated injections once. Each group was randomly divided into 6h, 12h, 24h3 subgroups at different time points after the rats were sacrificed by enzyme-linked immunosorbent assay (ELISA) detection of serum HMGB1 levels, and observed lung wet weight coefficient, oxygen changes in lung tissue pathology. Results 1.A group at each time point showed no significant change in HMGB1; B, C, D serum HMGB1 concentrations after 6h no significant change compared with no significant difference between group A; B, C, D group after 12h began to have significantly increased, 24h further increased, compared with the same time point of the A group, there was significant difference (P lt; 0.05). C, D group at 12h, 24h concentrations were significantly lower than the same point in time in group B (P lt; 0.05); D group, 12h, 24h concentrations were significantly lower than the same point in time in group C (P lt; 0.05). 2.C, D lung wet weight coefficient and lung pathology scores were significantly lower than conventional same time point in group B (P lt; 0.05); D group was significantly lower than the same point in time in group C (P lt; 0.05). Conclusion Xuebijing SAP can reduce lung injury in rats, the mechanism may be by regulating the levels of HMGB1 attenuates lung injury and therapeutic effect Xuebijing a dose-effect relationship.
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