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Background Numerous studies have demonstrated not only prolonged inhalation of high concentrations of oxygen can cause acute lung tissue damage, and can affect the further development of neonatal lung tissue, causing bronchopulmonary dysplasia (BPD) the occurrence of serious impact on the prognosis of newborns and quality of life, and the treatment of hyperoxia-induced lung injury is still no effective way. Xuebijing injection from safflower, Chuanxiong, red sage root, angelica, red peony, composed of pure Chinese medicine, used for sepsis, multiple organ dysfunction syndrome (MODS) treatment, and received a significant effect . Aim of the present study was to Xuebijing neonatal rats with hyperoxia-induced lung injury in rats. Pregnant 22 Skyway production childbirth method newborn SD rats within 12 hours after birth, number and randomly divided into six groups: Air saline group (AN group), air dexamethasone group (AD group), air Xuebijing (AX group), hyperoxia saline group (HN group), high oxygen dexamethasone group (HD group), Xuebijing hyperoxia group (HX group). Observation of neonatal rats with hyperoxia and mortality in general, and some lung wet / dry weight (W / D) ratio, lung HE staining; take part in bronchoalveolar lavage fluid (BALF) leukocyte count, testing protein content, LDH levels, TNF-α and IL-10 levels. Results 1. Mortality with hyperoxia exposure time, increased mortality of animals, Xuebijing can significantly reduce exposure to prolonged hyperoxia in neonatal rats 5 days and 7 days mortality (P <0.05). 2.BALF HN group in white blood cell count white blood cell counts were higher than the same time point AN group (P lt; 0.01). Dexamethasone in the hyperoxia group 3 and 5 days can reduce the hyperoxia group of abnormally elevated white blood cell count (P lt; 0.01), while Xuebijing can significantly reduce exposure to prolonged hyperoxia 5 days and 7 days BALE leukocytes numbers increased (P average lt; 0.01). 3.BALF high protein content of LDH and oxygen in BALF LDH and protein content is higher than in the same time point AN group (P lt; 0.05), dexamethasone, and Xuebijing can reduce hyperoxia 5 and 7 days in BALF LDH and protein content increased (P lt; 0.05). And with the experimental time, Xuebijing than dexamethasone to reduce the extent of more significant (P lt; 0.01). 4.BALF in TNF-α levels compared with the saline group air, HN group and the HD group in BALF TNF-α were significantly increased (P all lt; 0.01), dexamethasone and Xuebijing the hyperoxia group in BALF TNF-α were significantly lower (P all lt; 0.01), and the HX group decreased more significantly (P lt; 0.01). 5.BALF levels of IL-10 and AN group, hyperoxia group BALF levels of IL-10 was significantly lower (P all lt; 0.01), dexamethasone and Xuebijing the hyperoxia group compared to HN group, BALF of IL-10 was significantly higher (P all lt; 0.01), and the HX group increased more significantly (P lt; 0.01). 6.W / D ratio. In the last hyperoxia 5 and 7 days, HN group was significantly higher than the AN group and the AD group (P lt; 0.01). Dexamethasone reduces only sustained hyperoxia 7 days due to W / D ratio increased (P lt; 0.01), in the continuous hyperoxia 5 and 7 days, HX group was significantly lower than the HN group (P lt ; 0.01). 7 HE staining of lung tissue: Continuing hyperoxia three days, the lung tissue structural disorder, interstitial edema, alveolar seen a lot of red blood cells and inflammatory cells; sustained hyperoxia 5 days and 7 days, the lung tissue structure more disordered, lung interval widened, pulmonary interstitial cells increased. HX group can reduce lung inflammatory exudate, improve the structure of the lung tissue disorder 8. Radial alveolar count normal newborn rat lung tissue after birth RAC value gradually increased, in five days, seven days all time points, respectively, higher than the previous one respectively (P lt; 0.05). In the last three days after exposure to hyperoxia lung tissue RAC values ??were no significant differences in the air, with the prolonged hyperoxia inhibited RAC value increases to seven days after birth was significantly lower than the air control group (P lt; 001). Xuebijing point in time can be increased with the RAC value hyperoxia group (P lt; 0.01). Conclusion 1. Successfully prepared neonatal rats with hyperoxia-induced lung injury model. 2 hyperoxia newborn rat lungs can cause proinflammatory and antiinflammatory factor imbalance, causing acute lung injury and inhibits the normal development of the lung after birth. 3 of dexamethasone can improve pulmonary hyperoxia proinflammatory and antiinflammatory factor imbalance, reducing the extent of acute lung injury induced by hyperoxia, but can be further suppressed postnatal hyperoxia lung development. 4 Xuebijing better than the dexamethasone to improve pulmonary hyperoxia proinflammatory and anti-inflammatory cytokine imbalance is more effective in reducing the extent of acute lung injury induced by hyperoxia, and does not inhibit hyperoxia after birth lung development.
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