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Effects of Propofol on Lung Injury Induced by Hepatic Ischemia Reperfusion in Rats

Author: LiuRuZuo
Tutor: YangJiWu
School: Dali University
Course: Surgery
Keywords: Hepatic ischemia-reperfusion Lung injury Propofol Immunohistochemistry Rats
CLC: R965
Type: Master's thesis
Year: 2011
Downloads: 28
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Abstract

[Objective] To establish a rat hepatic ischemia-reperfusion (reperfusion of hepatic ischemia., HI / R) injury model intervention with propofol, the detection of liver tissue expression of TNF-α and NF-κB explore propofol on rat liver ischemia-reperfusion injury in the lung tissue protective effect. [Method] 48 healthy adult Sprague-Dawley (SD) rats were randomly divided into four groups: (1) sham group (Sham group, 6), only exposure to the hepatic portal, without any intervention, 30min later put to death; (2) ischemia group (group I, 6), the exposure of the first hepatic portal and occlusion of the hepatic pedicle, 30min later put to death; (3) ischemia-reperfusion model group (HI / R group, 18), clipping the hepatic pedicle 30 min before reperfusion via the femoral vein injection of saline 0.5ml as controls; propofol intervention group (Pro 18) (4), the occlusion of the hepatic pedicle 30min, perfusion through the femoral vein injection isopropyl phenol to 2mg/kg (diluted in 0.5ml of saline). (3) group, and (4) were in reperfusion after 30min, 60min, 120min time points each six rats were sacrificed. Put to death as soon as the lung tissue pathology research: lung tissue wet to dry weight ratio: immunohistochemical staining, detection of lung tissue TNF-α and NF-κB expression. Results of the application SPSS16.0 statistical software for statistical analysis and processing, Measurement data were expressed as mean ± standard deviation (χ ± s) said. Paired t test was used to compare the group, the groups were compared using one-way ANOVA, take the test level α = 0.05, P lt; 0.05 considered statistically significant. [Results] 1 the ordinary HE staining, sham group only exposed the first liver doors without occlusion of the hepatic pedicle, ischemia-reperfusion injury. Ⅰ part of the lung tissue showed congestion, edema. Alveolar space no obvious red blood cells and inflammatory cells. HI / R group lung tissue visible congestion, edema, and inflammatory cells and red blood oozing. With the infusion of extension of time, the injury gradually worsened. Pro group damage than the sham group and serious injury to a lesser extent compared with ischemia-reperfusion group. The damage trend with ischemia-reperfusion group similar. Lung tissue W / D results are shown: the W / D ratio of HI / R group at each time point are greater than the the Pro group corresponding time point, the sham group and I group the ratio of W / D (P lt; 0.01). Pro group at each time point W / D ratio in addition to less than the corresponding time points HI / R (P lt; 0.01), with group Ⅰ W / D ratio of comparison, the difference was not statistically significance (P gt; 0.05). Pro 30min and Pro 60min statistical results of the sham group no difference (P gt; 0.05). Lung tissue TNF-α immunohistochemical staining results showed that: (1) measured TNF-α levels in Ⅰ group and the sham group, the difference was no statistical significance (P gt; 0.05). (2) whether the HI / R group Pro group, TNF-α in the lung tissue from reperfusion 30min began gradually increased, but its value in HI / R group until 120min still on the rise, while in the Pro group in both values ??are 60min after reperfusion peaked. Ⅰ group TNF-α levels measured value is higher than the sham group, but no statistically significant results of the comparison. Pro group and HI / R group compared to the corresponding point in time Pro group lung TNF-α levels were lower than the HI / R group (P gt; 0.01) (3) of TNF-α content measured value in the comparison between groups in Pro 30min.Pro 120min, and I groups was not statistically significant (P gt; outside 0.05), HI / R group and PrO 60min TNF-α levels at all time points measured values ??were higher than group Ⅰ (P lt; 0.05). (4) TNF-α content measured value in the comparison between groups, Pro group at all time points measured values ??are less than the HI / R group. Pro group TNF-α levels measured value Pro 60min and Pro 120min sham group results was statistically significant (P lt; 0.05) Pro 60min Ⅰ group was statistically significant (P lt; 0.05). Pro 30min Pro 120min measured value Ⅰ group showed no statistical significance (P gt; 0.05), and the Pro 30min lung tissue TNF-α levels measured values ??with the sham group had no statistical significance. (5) HI / R group comparison, 60 min and HI HI / R / R 120min measured lung tissue TNF-α values ??with HI / R 30min difference was statistically significant (P lt; 0.05). The comparison between HI / R 60min and HI / R 120min result was not statistically significant (P gt; 0.05). (6) Pro group compared with the measured results showed no significant difference between the three time points. Ischemia-reperfusion TNF-α levels in the liver tissue of the model group from reperfusion 30min began to rise to reperfusion 60min peaked, then gradually decreased. Each time point ischemia-reperfusion content of TNF-α in the liver tissue of the model group to be significantly higher than the sham group, the difference between the two groups was significant (P lt; 0.05). Propofol interfere with the TNF-α content in the liver tissue with ischemia-reperfusion model group, but each time point the content of TNF-α in liver tissue against ischemia-reperfusion group, between the two groups significant difference (P lt; 005). Lung tissue NF-κB immunohistochemical staining results showed that: (1) I lung tissue content of NF-κB is higher than the sham group, below the HI / R group, and Pro group and HI / R group three The time point for statistical analysis, the comparison result of a significant difference (P LT; 0.01). NF-κB (2) HI / R group lung tissue content higher than the sham group I group and Pro group, its content from reperfusion 30min began to increase, and to reperfusion 60min peaked, then gradually decreased. (3) Pro lung tissue at all time points measured NF-κB content was significantly lower than the HI / R group corresponding point in time, within-group comparisons significant difference (P lt; 0.01), and each point in time trends with ischemia-reperfusion group the same. (4) HI / R group compared NF-κB values ??with HI / R 30min, 60min and 120min lung tissue difference was significant (P lt; 0.01), while the results of the comparison between 60min and 120min no statistics significance (P gt; 0.05). (5) Pro group comparison, the measured results at three time points between no statistical significance (P gt; 0.05). [Conclusion] hepatic ischemia reperfusion causes lung injury. Propofol has a protective effect on rat liver ischemia-reperfusion lung injury.

Effects of Propofol on Lung Injury Induced by Hepatic Ischemia Reperfusion in Rats

Abstract

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