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The Expression of GRP78 and Caspase-12 in Rats Sleep Deprivation During High-altitude Environment and Medicine Intervention

Author: MaYaJie
Tutor: YangJinSheng
School: Lanzhou University
Course: Neurology
Keywords: Sleep Deprivation Glucose-regulated protein Caspases Hippocampal
CLC: R965
Type: Master's thesis
Year: 2011
Downloads: 25
Quote: 0
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Abstract


Objective: To observe the dynamic changes of the hippocampus after GRP78 and caspase-12 in the high altitude environment of rapid eye movement sleep deprivation (SD) rats at different times and apoptosis of neurons ; 2 , using drugs ginsenosides Rd, ??observed the highland conditions sleep deprivation hippocampal GRP78 and caspase-12 expression , and to investigate the neuroprotective effect of ginsenoside Rd (GS Rd) may . Methods : adult Wistar rats 140 , male and female , weight control between 180-300g , provided by the Experimental Animal Center of Gansu Province, School of Medicine ; animals were randomly divided into two groups: of Lanzhou group and Hoh Xil group ; each group was randomly divided into a normal sleep group ( normal sleep n = 5) , GS Rd intervention group ( GSRd -1d , 3d, 5d ) and the saline control group (NS-1d, 3d, 5d), remove the drowning and accidental death after randomness supplemented rats , 5 mice each point in time to meet . This test uses classic platform the water environmental law making model of sleep deprivation in rats of different time points by immunohistochemical staining time point GRP78, Caspase-12 protein expression ; detected by TUNEL kit each time point cells withered death situation . Results: in Lanzhou group , compared with normal sleep , SDld rat GRP78 and Caspase-12 protein expression began to increase and reached a peak , 3d , 5d, no significant difference compared with the normal sleep group . GS Rd intervention group SDld 3d of GRP78 of expression than the saline group increased the SD5d of GRP78 expression and no significant differences in the saline group ; GS Rd intervention group the rat SD1d 3d caspase -12 expression is lower than the saline group Caspase-12 expression in the saline group , SD5d no significant difference. The Lanzhou group , at the same point in time Kekexili group GRP78 and Caspase - 12 expression were significantly increased. Conclusions : 1 . Endoplasmic reticulum stress initiate cell basic homeostatic regulation mechanism of such self - regulation , and the long - term and severe endoplasmic reticulum stress disappear , and induce apoptosis pathway induced apoptosis , which may be sleep deprivation one of the mechanisms that cause brain damage ; plateau environmental exposure may further exacerbate the degree of endoplasmic reticulum stress ; 3 . ginsenoside -Rd may be elevated GRP78 and reduce the expression of Caspase-12 protein levels to mitigate within endoplasmic reticulum stress , which play a cerebral protective effect .

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