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Oxidation is a necessary process for the life activities of the body, but too much oxidation cause the body to produce free radicals, resulting in the injury of biological macromolecules. In recent years, the study of oxidative stress and antioxidant has become the forefront of modern life sciences, and the focus of attention, evaluation and screening natural substances with antioxidant activity has been a new trend of biology, medicine, and food science. Lactic acid bacteria as probiotics has been widely applied to the food and medicine, there are a large number of studies have reported on the physiological activity of lactic acid bacteria at home and abroad. Recently, studies have reported anti-aging, anti-oxidation, such as lactic acid bacteria has important physiological functions, is an important resource for the development of natural antioxidants. This study was measured using DPPH method the four Lactobacillus rhamnosus B7, B8, B10, B44 of the antioxidant activity, analysis of the cell-free extract of each strain, such as whole cell and cell lysate component DPPH scavenging Its superoxide dismutase (SOD), glutathione peroxidase (GSH-PX), catalase (CAT) activity, and DPPH scavenging enzyme inactivation before and after each strain component comparison. Then screened according to the test results, an antioxidant activity relatively higher Lactobacillus rhamnosus strains continue and in vivo and in vitro antioxidant activity. According to alcoholism, the balance of pro-oxidants and antioxidants destruction, eventually leading to biological macromolecules such as fat, protein and DNA oxidative damage occurs, leading principle in the design of the cell injury in mouse models to prove that Lactobacillus rhamnosus antioxidant activity. Analysis of mouse plasma alanine aminotransferase (ALT), lipopolysaccharide (LPS), superoxide dismutase (SOD), malondialdehyde (MDA), as well as organizations in triglycerides (TG), free fatty acids, and cholesterol content to detect the protective effect of Lactobacillus rhamnosus strains of alcohol-induced oxidative stress. Ethanol and the Lactobacillus rhamnosus B10 processing Caco-2 cells, dihydro ethidium staining and immunoblot analysis of intracellular reactive oxygen species (ROS) and inducible nitric oxide synthase (NOS2) do The track detection analysis. The test results show that: the four Lactobacillus rhamnosus have a higher resistance to oxidation; contain superoxide dismutase (SOD), glutathione peroxidase (GSH-PX), catalase ( CAT) activity; the inactivated strains each component also has some antioxidant capacity. Relatively high antioxidant activity of strain B10 cell-free extract to scavenge DPPH was: 96.1%, 26.5% of the bacterial cells, 48.7% of the cell lysate. , Respectively, are higher than the other Lactobacillus rhamnosus strain. In vivo, after Lactobacillus rhamnosus strain B10 mice after plasma alanine aminotransferase (ALT), lipopolysaccharide (LPS), malondialdehyde (MDA) and liver triglyceride (TG) The free fatty acids and cholesterol were significantly lower than the concentration of the indicators of the alcohol group of mice. Plasma superoxide dismutase (SOD) activity was 0.44 ± 0.04 U / mg, significantly higher than the alcoholic mice 0.37 ± 0.02 U / mg. In vitro experiments, ethanol enable the Caco-2 cells to oxidative damage, staining and immunoblotting results show that Lactobacillus rhamnosus able to resist the ethanol-induced oxidative damage of the Caco-2 cells.
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