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Objective To study the sodium nitrite mechanisms of the protective effect and its role in ischemia-reperfusion injury in isolated perfused rat heart. Established Langendorff isolated rat heart model. 40 male Sprague-Dawley rats were randomly divided into five groups (n = 8), control group (C group): KH solution for isolated heart infusion 100min; ischemia-reperfusion group (I / R group): the first KH perfusion balance 40min, St.Thomas II stopped cardioplegia cardiac arrest 30min after reperfusion 60min and then KH solution; low, medium, high nitrite group (LN group, MN group, HN group): approach with I / R group, respectively, containing sodium nitrite (0.01mg / L, 0.1mg / L, 1mg / L) added the St.Thomas II cardioplegia. Before ischemia and reperfusion 10,30,60 min measured hemodynamic parameters and reperfusion 60min creatine kinase (CK) and lactate dehydrogenase (LDH) activity in the coronary effluent, myocardial tissue NO and NOS optical microscope observation of pathological changes of myocardium. Results of each group of cardiac function indicators baseline values ??(baseline) the difference was not statistically significant (P gt; 0.05); compared with baseline values ??at each time point CF I / R group re-perfusion, LVSP, dp / dtmax,-dp / dtmax decreased (P lt; 0.05); compared with C group, the LN group and the MN group at each time point CF, LVSP dp / _dtmax, -dp/_dtmax trend with the I / R group, but not as good as I / R group significantly (P lt; 0.05 or P lt; 0.01), the MN group downward trend is smaller than the LN group (P lt; 0.05), and the HN group at each time point CF, the LVS P dp / dtmax, dp / dtmax significantly low in the other groups (P lt; 0.01). Compared with C group, I / R group, LN group, MN group, HN group reperfusion 60min after coronary effluent CK and LDH were increased (P lt; 0.01), which LN group than in the the MN group increased trend significantly (P lt; 0.05), while the HN group coronary effluent CK and LDH was significantly higher than the other four groups (P lt; 0.01). I / R group at the end of reperfusion 60min LN group, MN group HN myocardial tissue homogenate NO content, NOS, iNOS and cNOS and group C were significantly increased (P lt; 0.05 or P lt; 0.01) LN group, MN Group the cNOS content higher than the I / R group (P lt; 0.05), the the MN group increased more significantly, the three groups iNOS content no difference (P gt; 0.05). The HN group iNOS significantly higher than the other four groups (P lt; 0.01) while the cNOS lower than the other four groups (P lt; 0.01). Light microscopy showed that the I / R group the myocardial injury lesions showed myocardial stripes and fuzzy, cell swelling, interstitial edema, vacuolar degeneration, see point flaky dissolved stove; application of sodium nitrite, LN group and MN myocardial tissue damage to mitigate the most obvious MN group, HN myocardial tissue damage present. Conclusion cardioplegic solution, adding an appropriate amount of sodium nitrite, can increase coronary blood flow, improve heart function after reperfusion of ischemic heart, to reduce CK and LDH levels, the protective effect on myocardial ischemia-reperfusion injury. Its mechanism of action may be induced with sodium nitrite cNOS synthesis, thereby reducing tissue ischemia-reperfusion injury.
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