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Objective: In recent years, with the deepening of the study of the mechanisms of spinal cord injury (SCI), the role of oxidative stress gradually aroused people's attention. and positive feedback persistence of oxidative stress [2]. Accordingly, and to explore the reduction or blocking the acrolein produce effective method is an effective method in the treatment of SCI. effectively remove acrylaldehyde the peroxide aldehyde [5]. Home and abroad on the LA role of oxidative stress in the spinal cord injury has not been reported. The study is to establish the rat model of spinal cord injury, the early application of LA, motor score was observed on functional recovery after spinal cord injury in rats, and by the thiobarbituric acid assay concentration from (malondialdehyde MDA), xanthine oxidase-peroxidase (SOD) activity, immunohistochemical method combined with computer image analysis technique semi-quantitative determination of the expression of acrolein (acrolein ACR), to further explore the protective effect of LA on spinal cord injury, clinical find effective measures and ways to treat spinal cord injury. Methods: health, adult Sprague-Dawley (SD) rats 66; weight 240-280g, mice aged 3-4 months, male or female. Using a modified Allen's-WD production model of spinal cord injury, 66 SD rats were randomly divided into normal control group of six, drug intervention group, 30 of spinal cord injury lipoic acid tail vein injection group, damage control group of 30 that spinal cord injury physiological tail vein injection of saline. 24 hours after spinal cord injury (h), 3 days (d), 7D, respectively, drawn, each group of eight, only HE staining and immunohistochemical measured acrolein expression, I directly derived by using spectrophotometer organization of SOD, MDA content. Damage control group and drug intervention group more than six rats were 3,7,14,21 d after SCI BBB motor score observed behavior changes. SPSS13.0 statistical software processing among the three groups of spinal cord tissue SOD, MDA measured values, ACR mean optical density and BBB Movement score the number of groups were compared using one-way ANOVA are a number of pairwise comparisons using LSD test. Results: 1 light microscope, the normal group neuron cell morphology, number, membrane integrity, unspoiled 24h see focal hemorrhage after spinal cord injury, neuronal cell swelling, round, reduce the number of perinuclear shrink, fragmentation, the Nissl bodies lightly stained or disappear. Lipoic acid treatment 24 hours after the visible neurons reduce swelling, increase in number than the injury group. 7d injury group, the number of neurons in the spinal cord injury was significantly reduced, and the formation of vacuoles. Treatment group neuronal morphology close to normal, swelling, necrosis, vacuolar changes significantly reduced compared with the control group. 24h and 7d treatment group spinal cord slices pathological changes of light than those in the control group. 2 Basso-Beattie-Bresnahan (BBB) ??behavioral score results: three groups in the preoperative BBB score there was no significant difference in P GT; 0.05, postoperative 3d, 7d, 14d, 21d at each time point injury group and lipoic acid treatment group were highly significant difference (p lt; 0.01), the treatment group at each time review scores significantly higher than the injury group; postoperative 3d, 7d, 14d, 21d each time point treatment group and the normal group had no significant difference (p GT ; 0.05) in the treatment group at each time review scores similar to the normal group, but did not reach normal. 3 SOD determination results, lipoic acid treatment group 24h, 3d, 7d of SOD activity values ??were 173.7 ± 6.9,221.1 ± 16.7,225.3 ± 13.8, significantly change over time increments. Injury group 24h, 3d, 7d SOD activity values ??were 124.7 ± 8.8,137.67 ± 4.8,145.9 ± 5.7 change over time increment is not obvious, two sets of three time points SOD activity values ??were highly significant differences (p lt; 0.01). First 3d, 7d treatment group and normal group (234.6 ± 11.9) showed no significant difference (p gt; 0.05), treatment group, SOD activity values ??close to normal. MDA determination results, lipoic acid treatment group 24h, 3d, 7d of MDA content were 7.43 ± 1.1,6.20 ± 0.71,5.85 ± 0.70, and descending change over time. Injury group 24h, 3d, 7d MDA content were 12.3 ± 1.2,11.9 ± 1.14,11.011 ± 1.09 change over time significant decrement three time points two groups of MDA content were highly significant differences (p lt; 0.01) treatment group at three time points MDA levels were significantly lower than the injury group. First 3d, 7d treatment group and normal group (5.5 ± 0.54) showed no significant difference (p gt; 0.05), MDA content close to normal in the treatment group. 5 spinal cord tissue acrolein immunohistochemical staining, lipoic acid treatment group 24h, 3d, 7d, the average optical density values ??were 0.2252 ± 0.0402,0.1965 ± 0.0435,0.1847 ± 0.0401, diminishing over time. The mean optical density of the injury group were 0.3636 ± 0.0100,0.3462 ± 0.0157,0.3052 ± 0.0162 a downward trend over time, the treatment group were significantly lower than in the average optical density value of the three time points injury group, three time points The mean optical density values ??were highly significant differences (p lt; 0.01). First 3d, 7d treatment group and normal group (5.5 ± 0.54) showed no significant difference (p gt; 0.05), treatment group, the average optical density values ??close to normal. Conclusion: This study further confirmed after spinal cord injury secondary lipid peroxidation products acrolein expression changes over time, and to verify the acrolein and free radical damage to the spinal cord injury in neuronal cells to participate. Powerful antioxidant lipoic acid can inhibit the the acrolein expression in the spinal cord injury and free radical scavenging, protect and promote the regeneration of endogenous antioxidant enzymes secondary to nerve injury, spinal cord injury yuan cells have a good The protective effect of a more significant role in promoting the recovery of motor function of the body.
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