Dissertation > Excellent graduate degree dissertation topics show

Initial Study on the Oral Candida Albicans with NMR-based Metabonomics

Author: ZouBin
Tutor: YangLan
School: Lanzhou University
Course: Clinical Stomatology
Keywords: metabolomics NMR Candida albicans PCA
CLC: R781.5
Type: Master's thesis
Year: 2011
Downloads: 96
Quote: 0
Read: Download Dissertation

Abstract


Candida albicans is a normal human body common bacteria, including oral carrier rate for the most part.Therefore,oral candidiasis is the most human oral fungal infection-prone diseases.With a variety of antibiotics,corticosteroids and immune agents such as drugs in clinical use. and diabetes, people with AIDS,organ transplantation and the increasing incidence of oral candidiasis rising trend year by year. Pathogens in the oral candidiasis Candida albicans in the most toxicand common. Candida albicans also detected in oral leukoplakia was 34%, and has now been confirmed Candida albicans infections associated with white spot prone tomalignant transformation. So for the identification of oral Candida albicans infection oforal fungal diseases and causes of oral cancer prevention research and treatment is very important. The current commonly used method for bacterial identification and moretime-consuming and labor-intensive, in the identification of the speed, accuracy and classification of oral bacteria, there are various ways inadequate.Metabolomics is developed in the past 20 years, low molecular weight metabolites of organisms quantitative and qualitative analysis of the new technology, with fast, efficient and quantitative characteristics.’H-nuclear magnetic resonance (1H-NMR) is one of the commonly used methods, it has the advantage that the sample to achieve non-destructive, non-selectivity of the analysis, and time-consuming very little. This method of cells by detecting an organ or a small molecular components of bacteria, obtained the NMR spectra of the sample. then the computer information technology on the NMR data obtained were analyzed to understand the biological significance of metabolite information. Metabolomics detection of oral Candida albicans have not been reported.In this study.1H-NMR-based metabonomics study of oral Candida albicans was detected in the extracellular metabolites to be characteristic of Candida albicans NMR spectrum, combined with principal component analysis of the map data were analyzed to explore the method for testing the feasibility of oral Candida albicans.Experiment consists of the following steps:1. Oral candidiasis in the culture. identification and growth curve mappingThe new issue of oral Candida albicans from patients gargle, the use of CHROMagar Candida cultures grown. Chromogenic medium identified by the bacteria inoculated purified by three generations of test tube slant, cold standby. Recovery of bacteria, the bacteria use the sand castle’s medium for culture growth curve.2. Obtain NMR spectra of Candida albicansBacterial culture will be the extracellular metabolites measured using nuclear magnetic resonance bacterial metabolites obtained 1H-NMR spectrum, access to the original free induction decay signal (FID) data. FID data obtained by magnetic resonance imaging and conversion software MESTREC points after treatment, storage, the next step analysis.3. The principal component analysisThe data will be used Simca-P software, principal component analysis method.Conclusion:The metabolism of oral Candida albicans obtained spectra, principal component analysis showed clustering of data between an internal focus, can be used to distinguish oral Candida albicans.1H-NMR-based metabonomics study the development of detection is a good prospect for the rapid detection method of oral Candida albicans.

Related Dissertations

  1. The Research of the Fault Diagnoses Algorithm for the Liquid Rocket Engine Testing Bed Based on PCA-SVM,V433.9
  2. Research on Recognition of Fabric Weave Pattern Based on Space-Frequency Domain,TS101.923
  3. The Research on Evaluation of Living Status Systems of Expressway Relocated People,D523
  4. Study on the Properties of Polyphosphatases from Beef Semitendinosus and the Enzymic Reaction Systems,TS251.1
  5. GC-MS Analysis of Endogenous Metabolites in Plasma of Police Dog after Aflatoxicosis,S858.292
  6. A Preliminary Study on Bare Soil Extraction and Its Moisture Retrieval from High Resolution SAR Data,S152.7
  7. Effective active ingredient with skullcap calixarene supramolecular inclusion complexes of,R284
  8. Oral epithelial cells were infected with Candida albicans after HBD-2 mRNA and protein expression in vitro,R780.2
  9. Application of Functional MR Imaging in Diagnose for Prostate Cancer,R737.25
  10. Image Restoration Method Research and Its Application Based on Probability PCA,TP391.41
  11. Design and Implementation of the Process Monitoring and Fault Diagnosis for Injection Molding,TQ320.5
  12. Research on Image Super-resolution Reconstruction Based on Non-local Similarity,TP391.41
  13. The Research on Neural Network with Quadratic Denominator Cubic Rational Spline Function Weight and Its Application,TP183
  14. NMR logging quadrature detection filter algorithm,TN911.23
  15. NMR logging sensor noise matching analysis and research,TP212
  16. Hash based on structured sparse spectrum image indexing algorithm,TP391.41
  17. Based on spectral regularization of linear dimensionality reduction methods,TP391.41
  18. Random Forests Feature Selection,TP311.13
  19. Based on PCA and SVM automotive coating line electromechanical equipment intelligent diagnosis,TH165.3
  20. Three-dimensional seismic signal density point number combination technology research,TP301.6
  21. Genotyping and Antifungal Susceptibility Testing of Candida Albicans from Patients with Atrophic or Erosive Oral Lichen Planus,R781.5

CLC: > Medicine, health > Oral Sciences > Oral medicine > Oral mucosal disease
© 2012 www.DissertationTopic.Net  Mobile