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Objective: To investigate the hypoxia-inducible factor -1a ( hypoxia - inducible factor - 1a , HIF -1a ) and stromal cell derived factor -1 (stromal cell-derived factor-1, SDF-1) in ischemic retinal diseases and retinal missing blood reperfusion ( retinal ischemia in - reperfusion expression pattern in the development of the RIR ) and the relationship of the two . Methods: 56 normal anterior and posterior section of adult SD rats were randomly divided into 0,6,12,24,48,72,144 h operation group and sham group , which the surgery group ( n = 42 ) , at each time point six only 14 sham group at each time point 2 , the application ligation rat carotid artery was established retinal ischemia reperfusion animal model . artery ligation Jin operation group after 60 minutes to restore blood flow perfusion , respectively, in the re- perfusion 0,6,12,24,48,72,144 h after the complete removal of the eye , cut off the cornea to remove products like body , clear the vitreous water the repeated slow flushing retina specimens , the the alcohol dehydration paraffin-embedded processing retinal specimens , using immunohistochemical The method and the mean optical density was detected at the same time relative to the group of retinal specimens HIF-1a and SDF-1 positive expression . Results: retinal ischemic reperfusion HIF-1a, SDF-1 did not express or slight expression in the sham group , the operation group HIF - 1a and SDF - 1 0h expression , 6h enhanced expression was strongest , 12h 24h decreased expression of the future with the passage of time expression decreased , the surgery group compared with the sham group difference was statistically significant ( P lt; 0.01 ) HIF - 1a and SDF-1 expression when consistent with a positive correlation. CONCLUSION: Hypoxia -inducible factor (HIF-1a) and stromal cell-derived factor (SDF-1) expression in retinal ischemia reperfusion retinal function is closely related to damage , both have a synergistic correlation .
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