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Silencing BAG-2 Gene Inhibits the Cell Proliferation of Human Lung Carcinoma A549 Cells

Author: JiaHaiTao
Tutor: HeQingZuo
School: Jinan University
Course: Genetics
Keywords: BAG - 2 RNA interference Lung adenocarcinoma Cell Proliferation Cyclin
CLC: R734.2
Type: Master's thesis
Year: 2011
Downloads: 36
Quote: 0
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Abstract


Lung cancer is the most common primary pulmonary malignancies , has become the world 's highest incidence of cancer . , Lung cancer accounts for about 25 % of primary lung cancer in recent years, the incidence rate is also rising . In this study, differences in subcellular proteomics technology to filter out the high expression in A549 cells Bag - 2 protein , and their biological functions in cancer research . Objective: To observe silence BAG- 2 gene on the cell cycle and proliferation of cells of lung adenocarcinoma cell line A549 ; explore the targeted RNA interference BAG- 2 protein on the proliferation ability of human lung adenocarcinoma cell line A549 via the ubiquitin - proteasome via regulation of cell cycle proteins . Methods: The human lung adenocarcinoma cell line A549 as the research object , transient transfection of specific BAG - 2 siRNA , the role of A549 cells at 0, 24, 48 h after western blot detection BAG- 2 protein levels silencing effect ; transfection BAG -2 siRNA 48 h after flow cytometry cell cycle ; the CFSE - active dye into A549 cells transfected with BAG-2 siRNA for 48 h and 72 h after the change of flow cytometry cell proliferation ; Western blot detection BAG- 2 siRNA role A549 cells 48 h after cell cycle protein expression changes . Results : 50 nM BAG-2 siRNA role A549 cells after 24, 48, h , BAG - 2 protein expression were significantly reduced ; BAG - 2 siRNA role in A549 cells after 48 h , cell cycle no significant change compared with the control group ; BAG - 2 siRNA A549 cells , cell proliferation was detected after 48 h and 72 h , the experimental group cell proliferation compared with control group proliferation slow ; Western blot detection BAG- 2 siRNA role in A549 cells after 48 h of Cyclin B1 expression level changes in human found that compared with the control group , the cell cycle protein cyclin B1 downregulation . Conclusion: The targeted BAG - 2 RNA interference technology , found silence BAG - 2 gene can inhibit the proliferation of lung adenocarcinoma cell line A549 , may be by affecting the ubiquitin - proteasome via regulate the cell cycle protein , thereby regulating cell proliferation .

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CLC: > Medicine, health > Oncology > Respiratory system tumors > Lung tumors
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