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Objective: colorectal cancer (colorectal cancer, CRC), including colon and rectal cancer, is a common gastrointestinal malignancy. Autophagy (autophagy) cells in the regulation of autophagy-related genes (autophagy related gene, Atg) use of lysosomal digestive degradation itself \metabolic cycle of cells and organelles updates provide raw materials and energy, it is the cells adapt to internal and external environment required a basic regulation [1]. Of this study was to detect autophagy markers of Beclin1 and p62 expression in colorectal cancer tissues and normal mucosa, to determine colorectal cancer tissues and normal mucosa autophagy activity. Combined with clinical and pathological data were analyzed to explore the occurrence of autophagy and colorectal cancer, the development of the relationship between the invasion and metastasis. Methods: Fourth Clinical Hospital of Hebei Medical University, outside the Second Division in October 2009 to October 2010, the surgical removal of 32 cases of patients with colorectal tumor tissue and normal mucosal tissue 19 cases (taken from the tumor tissue 5cm outside the intestinal mucosa) . Tissues were fixed in 4% paraformaldehyde, conventional dehydration, paraffin-embedded, 4μm thick serial sections. By immunohistochemical staining analysis of Beclin1 and p62 expression activity and tumor pathological type, depth of invasion, lymph node metastasis and occurrence site index correlation. Results: 1 tumor p62 protein expression level rise: The positive expression rate of P62 protein in colorectal cancer and normal mucosa were 81.25%, 47.36%, and the two groups was statistically significant (P lt; 0.05). Beclin1 protein in colorectal cancer tissues and normal mucosal tissue positive expression rate of 71.88%, 78.94%, and the two groups had no statistical significance (P gt; 0.05). 2 Beclin1, p62 expression levels and tumor pathological types: positive expression rate of Beclin1 in protein in the tubular adenocarcinoma and mucinous adenocarcinoma were 68.97%, 100%, showed no statistical significance (P gt; 0.05). The positive expression rate of p62 protein in tubular adenocarcinoma and mucinous adenocarcinoma were 82.76%, 66.67%, and the two groups had no statistical significance (P gt; 0.05). Beclin1 in p62 expression and colorectal cancer invasion depth: Beclin1 protein in tumors not infiltrating the serosa infiltration serosa positive expression rate was 66.67%, 73.91%, and the two groups was statistically significant (P gt; 0.05). p62 protein in the tumor did not serosal infiltration serosa positive expression rate were 66.67%, 86.96%, and the two groups had no statistical significance (P gt; 0.05). 4 Beclin1, p62 expression and colorectal cancer lymph node metastasis: Beclin1 protein positive expression rate of lymph node metastasis and without lymph node metastasis were 75%, 68.75%, and the two groups had no statistical significance (P gt; 0.05) . p62 protein positive expression rate of lymph node metastasis and without lymph node metastasis were 87.50%, 75%, showed no statistical significance (P gt; 0.05). 5 Beclin1, p62 expression and colorectal cancer occurs unrelated to the location: The positive expression rate of Beclin1 in protein in colon and rectal cancer were 61.11%, 85.71%, and the two groups had no statistical significance (P gt; 0.05). The positive expression rate of p62 protein in colon and rectal cancer were 92.30%, 73.68%, and the two groups had no statistical significance (P gt; 0.05). 6 Beclin1 and p62 expression in colorectal no correlation (P gt; 0.05). Conclusion: colorectal cancer tissues autophagy activity decreased. 2 the autophagy activity colorectal pathological type, depth of invasion, lymph node metastasis and tumor unrelated.
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