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Expression of GRIM-19 and STAT3 in Hepatocellular Carcinoma Tissues and Its Significance

Author: FengQiZhu
Tutor: ZhangChao；XiongQiRu
School: Anhui Medical University,
Course: Surgery
Keywords: Hepatocellular carcinoma Combination of interferon / retinoic acid -induced apoptosis related genes 19 Signal transducer and activator of transcription 3 is Immunohistochemistry
CLC: R735.7
Type: Master's thesis
Year: 2011
Downloads: 32
Quote: 0
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Abstract


Background liver cancer is a high incidence of malignant tumors in China, widely distributed, transfer rate, the molecular mechanism is not very clear. Recent studies suggest that the incidence of liver cancer have a certain relationship with the activation of certain oncogenes and inactivation of tumor suppressor genes, and the prevention and treatment of liver cancer from gene level theory, provide a vision for the treatment of liver cancer. Of GRIM-19 (gene associated with retinoid-IFN-induced mortality-19) is a retinoic acid / interferon induce apoptosis related genes in a combination, by Angell such as screening out a new apoptosis-related genes. GRIM-19 is located on human chromosome 19p13.1, constituted by 144 amino acids has a molecular weight of 16 KD protein and STAT3-specific binding occurs, blocking the cytoplasm of STAT3 entering the nucleus, thereby reduced STAT3 transcriptional activation activity, inhibiting STAT3 downstream target gene expression and cell growth. In addition, in the process of infection lead to carcinogenesis, GRIM-19 may be the target viral oncogene binding interactions can also protein NOD2, GW112, etc.. High expression of GRIM-19 can increase the sensitivity of cells to interferon in combination with retinoic acid-induced apoptosis, thereby inhibiting tumor cell proliferation and promote apoptosis. Domestic and foreign research reveals GRIM-19 expression in primary renal cell carcinoma, the urinary system tumors, lung cancer, and ovarian cancer tumors are missing or seriously reduce colorectal cancer, lung cancer, and ovarian cancer, further research found GRIM-19 gene at the mRNA and protein levels significantly lower than the control group, while STAT3 gene expression was significantly higher. However on GRIM-19 and STAT3 expression in liver cancer and the relationship with the development of liver cancer remains unclear. The purpose of detection of GRIM-19 and STAT3 expression in hepatocellular carcinoma, cancer and peripheral tissues, hepatitis cirrhosis organizations and normal liver tissue, comparison of GRIM-19 and STAT3 relationship and significance in the development of hepatocellular carcinoma . Explore the clinical and pathological data correlation of GRIM-19 and STAT3 with hepatocellular carcinoma pathological type, AFP, and portal vein tumor thrombus. Methods Immunohistochemistry Streptomyces GRIM-avidin - peroxidase (SP) method detected 31 cases of hepatocellular carcinoma tissue and corresponding adjacent tissues, 20 patients with hepatitis after cirrhosis and 10 patients with normal liver tissue 19 and STAT3 expression. Results 1.GRIM-19 positive expression in hepatocellular carcinoma was 48.3% (15/31), the positive expression in cancer tissues was 64.5% (20/31), 80.0% positive expression in the cirrhotic liver tissue ( 16/20), positive expression in normal liver tissue was 40.0% (4/10), the difference was statistically significant (p <0.05) in hepatocellular carcinoma and cirrhosis of the liver tissue by the statistical test GRIM-19 protein expression; of GRIM -19 protein expression difference was statistically significant (p <0.05) in cirrhosis of the liver tissue and normal liver tissue. GRIM-19 protein expression in HCC tissue and noncancerous liver tissue and noncancerous liver tissue and cirrhosis of the liver tissue differences were not statistically significant (p gt; 0.05). 2.STAT3 expression of hepatocellular carcinoma was 67.7% (21/31), the positive expression in the surrounding tissues was 41.9% (13/31), the positive expression in the cirrhotic liver tissue was 35.0% (7/20) in the positive expression of normal liver tissue was 20.0% (2/10), statistical tests of STAT3 expression in hepatocellular carcinoma was significantly higher than in noncancerous liver tissue, cirrhosis tissues of normal liver tissue (p <0.05); STAT3 protein expression in noncancerous liver tissue, cirrhosis of the liver tissue and normal liver tissue showed no significant difference (p gt; 0.05). There was no difference between the 3.GRIM-19 positive expression of gender, age, tumor size, HBsAg and portal vein invasion (p gt; 0.05), while the related GRIM-19 expression in hepatocellular carcinoma with tumor differentiation, GRIM- 19 expression in well-differentiated hepatocellular carcinoma was significantly higher than poorly differentiated hepatocellular carcinoma (p lt; 0.05); positive expression of STAT3 gender, age, tumor size, tumor differentiation, HBsAg, AFP and portal vein violated no obvious correlation. 4. Associated χ2 test analysis showed that GRIM-19 and STAT3 expression in hepatocellular carcinoma has a significant negative correlation (r = -0.468, p lt; 0.05). Concluding hepatocellular carcinoma STAT3 sustained high expression of GRIM-19 low expression of coexistence, GRIM-19 and STAT3 may exist specific binding interactions involved in the process of cirrhosis, hepatocellular carcinoma occurred; GRIM- 19 as a new apoptosis-promoting genes, may become a new tumor markers can be used for the early screening of hepatocellular carcinoma.

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CLC: > Medicine, health > Oncology > Gastrointestinal Cancer > Liver tumors
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