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Association of a Single Nucleotide Polymorphism in the CTR Gene with Osteoarthritis

Author: GuoAnYun
Tutor: JiangQing
School: Nanjing University
Course: Surgery
Keywords: CTR OA Urolithiasis Single nucleotide polymorphism Taqman probe
CLC: R684
Type: Master's thesis
Year: 2011
Downloads: 37
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Abstract


Objective:Taqman probe for the determination of single nucleotide polymorphisms has been very mature technology that can be used in many specific areas of single-nucleotide polymorphism analysis. Now, is is found that the incidence of OA in the patients with Urolithiasis is relatively lower than the general population, while the incidence of Urolithiasis in patients with OA is also lower than the general population. From our study we know that CTR gene can affect calcium metabolism and thus affect the incidence with OA and Urolithiasis related to the CTR gene 1377bp base C/T polymorphism is the most common. In this study, we learn the CTR gene 1377bp nucleotide sequence C/T polymorphism by Taqman probe, to analyze the relationship between which and the incidence of OA, to further explore the relationship pathogenesis of OA and Urolithiasis may existMethods:In this study, we use three groups of DNA extracted from oral swabs. Three groups were derived from OA patients, urinary stone patients and healthy subjects. The group of OA patients and Urolithiasis patients are the experimental groups, while normal group is control group. In our experiment, Taqman probe is used for SNP analysis. We use 10μl system. Different genotypes are located in different coordinate system after RT-PCR,. Then we can count the number of each group and do consequent result analysis.Results:Through the statisticsdistribution of CC, CT, TT genotype and C, T frequency in group Urolithiasis and group OA are not different significant (P>0.05), the statisticsdistribution between OA group ang group control are of difference (P<0.05). What’s more the frequency of CC, CT, TT genotype and allele C, T in group Urolithiasis are fit for the number reported in the literature. Contrastd with control group, the CT and TT genotypes are significantly higher in group Urolithiasis ang group OA, and the frequency of allele T is also higherConclusion:There is relationship between CTR gene 1377bp single nucleotide C/T polymorphism and the incidence of OA and Urolithiasis, and allele T may be pathogenic. But the out-patient records clearly suggests that a low incidence of Urolithiasis in patients with OA, and a low incidence of Urolithiasis in patients with OA. The survey found that incidence of Urolithiasis in female is lower than male while it is inversed in OA, especially for postmenopausal women, the incidence of OA is significantly higher.In our outpatient department, the patients of Urolithiasis are mostly male and OA are female, that may be the reason the incidence of Urolithiasis has a negative correlation with incidence of OA. By which, we can explain why there is no significant differentiation in group Urolithiasis and group OA in the CTR gene 1377bp single nucleotide C/T polymorphism in our results. The root cause of the phenomenon that the incidence of Urolithiasis and OA are of negative correlation is the different of sex but not genotype, or it is not CTR gene 1377bp single nucleotide C/T polymorphism at least.

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CLC: > Medicine, health > Surgery > Orthopaedic Surgery ( movement system diseases,orthopedic surgery ) > Joint disease and injury
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